Anti-PCYT2抗体
参阅全部 PCYT2 一抗
兔多克隆抗体to PCYT2
Rabbit
适用于: ICCmore details
与反应: Human
预测可用于: Cow, Non human primates
Synthetic peptide corresponding to Human PCYT2 aa 157-172.
Sequence:
TKAHHSSQEMSSEYRE
(Peptide available as ab39790)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 99% Tris glycine, 0.1% BSA
浓度
50 µg 浓度为 0.4 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15053于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC | Use a concentration of 5 µg/ml. |
Entrez Gene: 5833 Human
SwissProt: Q99447 Human
Unigene: 569843 Human
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Immunocytochemistry - Anti-PCYT2 antibody (ab15053)
Immunofluorescence analysis of PCYT2 in Hep G2 cells using ab15053. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab15053 at 5 µg/mL in 0.1% BSA and incubated incubated at 4 degree Celsius overnight followed by a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody at 2µg/ml. (Panel d) represents the merged image showing co-localization of PCYT2 with GRP94 in Endoplasmic Reticulum. (Panel e) shows the no primary antibody control. The images were captured at 60X magnification.
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