Anti-Histone H2A (phospho S129)抗体
参阅全部 Histone H2A 一抗
兔多克隆抗体to Histone H2A (phospho S129)
Rabbit
适用于: WB, ELISA, PepArrmore details
与反应: Saccharomyces cerevisiae
不与反应: Human
Synthetic peptide corresponding to Saccharomyces cerevisiae Histone H2A aa 100 to the C-terminus (phospho S129) conjugated to bovine serum albumin.
Database link: P04911
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15083于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | 1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
| ELISA | Use at an assay dependent concentration. | |
| PepArr | Use a concentration of 0.2 - 0.02 µg/ml. |
SwissProt: P04912 Saccharomyces cerevisiae
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Western blot - Anti-Histone H2A (phospho S129) antibody (ab15083)
All lanes : Anti-Histone H2A (phospho S129) antibody (ab15083) at 1/500 dilution
Lane 1 : S.cerevisiae yeast extract (SCYE) + control peptide with Human Histone H2A peptide (ab19751)
Lane 2 : S.cerevisiae yeast extract with 0.2 % Methyl methanesulfonate (1 hour) with Human Histone H2A peptide (ab19751)
Lane 3 : SCYE + phospho peptide with Histone H2A peptide - phospho S129 (ab19828)
Lane 4 : SCYE_M + phospho peptide with Histone H2A peptide - phospho S129 (ab19828)
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 14 kDa
The blots were produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membranes were then blocked for an hour. 1 microgram per mL of control- (ab19751, lane 1 and 2) or phospho-peptides (ab19828, lane 3 and 4) were added to the primary antibody ab15083 (rabbit anti-Histone H2A (phospho S129) antibody; diluted 1:500) and loading control ab125247 (mouse anti-GAPDH antibody; diluted 1:20000) and the membranes were incubated with peptide/antibody mixture for 24 hours at 4°C. Antibody binding was detected using infrared (IR)-labelled goat anti-rabbit (green) and IR-labelled goat anti-mouse (red; insert below) antibodies, diluted 1:20,000, for 1 hour at room temperature before imaging.
ELISA - Anti-Histone H2A (phospho S129) antibody (ab15083)
Serially diluted ab15083 was bound to immobilised phospho (ab19828) - or control (ab19751) peptides (1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed with TMB substrate.
Western blot - Anti-Histone H2A (phospho S129) antibody (ab15083)
Anti-Histone H2A (phospho S129) antibody (ab15083) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
Peptide Array - Anti-Histone H2A (phospho S129) antibody (ab15083)
All batches of ab15083 are tested in Peptide Array against peptides to different Histone H2A modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H2A - phospho S129 (ab19828), indicating that this antibody specifically recognises the Histone H2A - phospho S129 modification.
ab19828 - Histone H2A - phospho S129
ab19751 - Histone H2A - unmodified
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