Anti-LYVE1抗体- BSA and Azide free
参阅全部 LYVE1 一抗
兔多克隆抗体to LYVE1 - BSA and Azide free
Rabbit
适用于: ICC, IHC-Pmore details
与反应: Mouse
Recombinant fragment (His-tag) within Mouse LYVE1 aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. C-terminal His tag
Database link: Q8BHC0
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: PBS
是
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
Protein-A Chromatography (+his tag depleted).
多克隆
IgG
Abpromise™承诺保证使用ab14917于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| IHC-P | (27) | Use at an assay dependent concentration. |
Entrez Gene: 114332 Mouse
SwissProt: Q8BHC0 Mouse
Unigene: 396078 Mouse
CRSBP1 antibody
extracellular link domain containing 1 antibody
extracellular link domain-containing 1 antibody
Extracellular link domain-containing protein 1 antibody
HAR antibody
Hyaluronic acid receptor antibody
Lymphatic endothelium specific hyaluronan receptor antibody
lymphatic vessel endothelial hyaluronan receptor 1 antibody
Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
LYVE 1 antibody
LYVE-1 antibody
LYVE1 antibody
LYVE1_HUMAN antibody
XLKD1 antibody
Cell surface retention sequence-binding protein 1 antibody
CRSBP 1 antibody
CRSBP-1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)
Immunohistochemical analysis of paraffin-embedded mouse intestine tissue staining LYVE-1 with ab14917. Positive staining is shown in the lymphatic endothelial cells (red).
Immunocytochemistry - Anti-LYVE1 antibody - BSA and Azide free (ab14917)
Immunocytochemistry/immunofluorecent analysis of mouse colon tissue labelling LYVE-1 with ab14917 (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)Divan et al PLoS One. 2018 May 3;13(5):e0196893. doi: 10.1371/journal.pone.0196893. eCollection 2018. Fig S1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
At the time point of 75dpi spirochetes (Bb) were not observed in association with the lymphatic-like vessels (LYVE-1) that run parallel to the sagittal sinus (SS, arrow) of the dura mater.
Dura samples were collected from transcardially perfused mice by craniotomy and post-fixed in 4% paraformaldehyde for 24h at 4°C. Samples were permeabilized in 0.1% Triton X-100, washed 3 times, and serum-blocked in 2.5% goat serum/PBS containing 1:100 dilution of Fc block. For B. burgdorferi staining, each sample was incubated in 1:100 dilution of rat anti-mouse unconjugated monoclonal anti-CD31 IgG, and 1:50 dilution biotinylated rabbit anti-B. burgdorferi polyclonal IgG at 4°C overnight. On the following day, the samples were washed, and stained with 1:100 dilution of Alexa 555 goat anti-rat polyclonal IgG, and 1:200 dilution of Alexa 488 streptavidin for 1 hour at room temperature, covered from light. Secondary antibody-only controls for B. burgdorferi indirect fluorescent assay were performed in vitro and no fluorescence was observed.
Some of the dura samples were also stained for lymphatic vessels in a separate step, using 1:200 ab14917, followed by washing and secondary staining with 1:200 Alexa 633 goat-anti rabbit polyclonal IgG (yellow).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)This image is courtesy of an Abreview submitted by Elizabeth Chlipala on 18 November 2005.
ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)Image is courtesy of an anonymous Abreview
ab14917 staining LYVE1 in mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a EDTA-buffer pH 9.0. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 20°C. A biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)This image is courtesy of an anonymous Abreview
ab14917 at 1/2000 dilution staining Ha-Ras transgenic mouse bladder (cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the primary antibody for 12 hours. A biotinylated polyclonal antibody was used as the secondary.
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