Anti-iNOS antibody

产品名称

Anti-iNOS抗体
参阅全部 iNOS 一抗

描述

兔多克隆抗体to iNOS

宿主

Rabbit

特异性

The immunogen used to raise this antibody shares 84% homology with Rat iNOS.

经测试应用

适用于: IHC-P, WBmore details

种属反应性

与反应: Mouse, Rat, Recombinant fragment

免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

阳性对照

常规说明

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid

存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

存储溶液

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

纯度

Immunogen affinity purified

克隆

多克隆

同种型

IgG

he Abpromise guarantee

Abpromise™承诺保证使用ab15323于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

别名

Western blot - Anti-iNOS antibody (ab15323)

All lanes : Anti-iNOS antibody (ab15323) at 1 µg/ml

Lane 1 : Raw264.7
Lane 2 : Raw264.7 + LPS (1 ug per mL; 18 hours)

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : IR- goat anti-rabbit (green) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size: 131 kDa

This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system under denaturing, reducing conditions. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with rabbit polyclonal to iNOS (ab15323; 1 ug x mL-1 (based on antibody concentration of 0.2mg/ml - lot GR126616-1) and the loading control mouse anti-GAPDH antibody (ab8245; 1:10000) overnight at 4°C. Antibody binding was detected using infrared (IR) labelled goat anti-rabbit (green; 1:10000) and IR-goat anti-mouse (red; 1:10000) for 1 hour at room temperature before imaging.

Western blot - Anti-iNOS antibody (ab15323)

All lanes : Anti-iNOS antibody (ab15323) at 5 µg/ml

Lane 1 : Raw 264.7 whole cell lysate + PMA
Lane 2 : Raw 264.7 whole cell lysate + PMA + LPS + Brefeldin A

Lysates/proteins at 20 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 131 kDa
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.


Exposure time: 8 minutes

Western blot - Anti-iNOS antibody (ab15323)

All lanes : Anti-iNOS antibody (ab15323) at 5 µg/ml

Lane 1 : Human recombinant iNOS protein
Lane 2 : Mouse recombinant iNOS protein

Lysates/proteins at 0.1 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 131 kDa


Exposure time: 1 minute

Membrane was blocked in 2% milk for 1 hour at RT. Primary anitbody was incubated at 4°C overnight.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody (ab15323)

IHC image of iNOS staining in a section of formalin-fixed paraffin-embedded normal mouse lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15323, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody (ab15323)

IHC image of iNOS staining in a section of formalin-fixed paraffin-embedded normal rat lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15323, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.