Anti-Claudin 1抗体
参阅全部 Claudin 1 一抗
兔多克隆抗体to Claudin 1
Rabbit
适用于: ICC/IF, WB, IHC-Pmore details
与反应: Human
预测可用于: Mouse, Rat, Dog, Monkey
Synthetic peptide within Human Claudin 1 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O95832
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
浓度
1 ml 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15098于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) | Use a concentration of 5 µg/ml. |
| WB | (6) | Use at an assay dependent concentration. Predicted molecular weight: 23 kDa. |
| IHC-P | (10) | Use at an assay dependent concentration. |
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Unigene: 289441 Mouse
Unigene: 24293 Rat
Entrez Gene: 9076 Human
Entrez Gene: 12737 Mouse
Omim: 603718 Human
SwissProt: O95832 Human
SwissProt: O88551 Mouse
Unigene: 439060 Human
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Western blot - Anti-Claudin 1 antibody (ab15098)
Anti-Claudin 1 antibody (ab15098) at 1/25 dilution + HeLa cell lysate
Predicted band size: 23 kDa
Observed band size: 19 kDawhy is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody (ab15098)
ab15098 (1µg/ml) staining Claudin 1 in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cell membrane.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody (ab15098)
ICC/IF image of ab15098 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15098, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
