Anti-Slit1抗体
参阅全部 Slit1 一抗
兔多克隆抗体to Slit1
Rabbit
This affinity purified antibody is directed against mouse SLIT-1 protein. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-1 from human, chicken and frog sources, as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). Cross reactivity with SLIT-1 homologues from other sources has not been determined.
The smaller strong bands observed in the WB shown are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).
适用于: WBmore details
不适用于: IHC-P
与反应: Mouse, Rat
Synthetic peptide corresponding to Mouse Slit1 aa 450-550 conjugated to keyhole limpet haemocyanin.
Database link: Q80TR4
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1.2 mg/ml
Immunogen affinity purified
This affinity purified antibody is directed against mouse SLIT-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Abpromise™承诺保证使用ab10984于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500. Detects a band of approximately 168 kDa (predicted molecular weight: 168 kDa). |
应用说明
Is unsuitable for IHC-P.
Entrez Gene: 20562 Mouse
SwissProt: Q80TR4 Mouse
Unigene: 40322 Mouse
Unigene: 30002 Rat
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Western blot - Anti-Slit1 antibody (ab10984)
ab10984-Rabbit polyclonal to Slit1 (1/500)
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000); 20µg of cell lysate per lane. Expected molecular weight: 168 kDa; Exposure time: 5 min
Lane 1: Rat Brain tissue lysate
Lane 2: Mouse Brain tissue Lysate
Note: The smaller strong bands observed in this WB are likely to be cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kDa -160kDa, (Little et al, 2002).
ab10984-Rabbit polyclonal to Slit1 (1/500) Secondary ab: Goat anti-rabbit HRP ab6721 (1/5000); 20µg of cell lysate per lane. Expected molecular weight: 168 kDa; Exposure time: 5 min Lane 1: Rat Brain tissue lysate Lane 2: Mouse Brain tissue Lysate Note: The smaller strong bands observed in this WB are likely to be cleavage products. A number of
