Anti-KAP1抗体
参阅全部 KAP1 一抗
兔多克隆抗体to KAP1
Rabbit
适用于: WB, IHC-P, IPmore details
与反应: Mouse, Rat, Human
Synthetic peptide. The immunogen is between aa 1-50.
Database link: Q13263
WB: human HeLa, HEK293T cells; mouse NIH3T3, TCMK-1, 4T1, CT26.WT, rat C6 cells. IP: HeLa cells. IHC: human breast carcinoma
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab10483于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | 1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa). |
| IHC-P | (1) | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| IP | (1) | Use at 2-10 µg/mg of lysate. |
Entrez Gene: 10155 Human
Entrez Gene: 21849 Mouse
Omim: 601742 Human
SwissProt: Q13263 Human
SwissProt: Q62318 Mouse
Unigene: 467408 Human
Unigene: 15701 Mouse
Unigene: 398345 Mouse
Unigene: 198494 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab10483)
Immunohistochemistry analysis of formalin-fixed paraffin embedded sections of human breast tissue staining KAP-1 with ab10483 at 1/1000 dilution, showing the current lot on the left, and previous lot on the right.
Immunoprecipitation - Anti-KAP1 antibody (ab10483)
Immunoprecipitation of KAP-1 from HeLa whole cell lysate using ab10483
Lane 1: ab10483 current lot
Lane 2: ab10483 previous lot
Lane 3: other rabbit anti-KAP-1 antibody
Lane 4: Control IgG
Western blot - Anti-KAP1 antibody (ab10483)
All lanes : Anti-KAP1 antibody (ab10483) at 1 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 3 : 4T1 (Mouse mammary gland carcinoma cell line) whole cell lysate
Lane 4 : CT26.WT (murine colon carcinoma) whole cell lysate
Lane 5 : C6 (rat glioma cell line) whole cell lysate
Lysates/proteins at 50 µg/ml per lane.
Predicted band size: 100 kDa
Exposure time: 3 minutes
Western blot - Anti-KAP1 antibody (ab10483)
All lanes : Anti-KAP1 antibody (ab10483) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK293T cells (human epithelial cell line from embryonic kidney transformed with large T antigen) at 50 µg
Predicted band size: 100 kDa
Exposure time: 30 seconds
Immunoprecipitation - Anti-KAP1 antibody (ab10483)This image was taken from an abreview submitted by an Seth Frietze.
The ab10483 antibody was used to immunoprecipitate KAP1 from HEK293 nuclear extracts. Three different amounts of ab10483 or control IgG were tested (1, 2 or 4 ug). The eluates from these experiments as well as the supernatants from the KAP1 IPs were analyzed by Western blotting using the ab10483 antibody. As shown by Western blotting, the presence of a ~110 kDa band demonstrated that KAP1 was specifically precipitated from these extracts. The 50 and 25 kDa bands correspond to the IgG of the IP antibodies.
