Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)]

• 产品名称

  Anti-CD1d抗体[NOR3.2 (NOR3.2/13.17)]
  参阅全部 CD1d 一抗

• 描述

  小鼠单克隆抗体[NOR3.2 (NOR3.2/13.17)] to CD1d

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  预测可用于: Sheep

• 免疫原

  Fusion protein corresponding to Human CD1d.

• 阳性对照

• IHC-P: Human skin tissue. Flow Cyt (Intra): MOLT4 cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 纯度

  Affinity purified

• 纯化说明

  Purified from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  NOR3.2 (NOR3.2/13.17)

• 骨髓瘤

  NS0

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab11076于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 912 Human

• Omim: 188410 Human

• SwissProt: P15813 Human

• Unigene: 1799 Human

• 别名

• CD1d antibody

• CD1D antigen antibody

• CD1D antigen d polypeptide antibody

• CD1d molecule antibody

• CD1D_HUMAN antibody

• Cd1d1 antibody

• differentiation antigen CD1 alpha 3 antibody

• HMC class I antigen like glycoprotein CD1D antibody

• Ly 38 antibody

• MGC34622 antibody

• R3 antibody

• R3G1 antibody

• T cell surface glycoprotein CD1d antibody

• Thymocyte antigen CD1D antibody

• Antigen-presenting glycoprotein CD1d antibody

• CD1.1 antibody

• CD1A antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

  IHC image of CD1d staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab11076, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Flow Cytometry (Intracellular) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

  Overlay histogram showing MOLT4 cells stained with ab11076 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11076, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabeled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)Image courtesy of an anonymous Abreview.

  ab11076 staining CD1d in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11076 at a 1/200 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit IgG.