Anti-UCP3抗体
参阅全部 UCP3 一抗
兔多克隆抗体to UCP3
Rabbit
适用于: IHC-Pmore details
与反应: Human
Synthetic peptide corresponding to Human UCP3 aa 254-267 conjugated to keyhole limpet haemocyanin.
Database link: P55916
Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 0.1% BSA
浓度
50 µl 浓度为 1.1 mg/ml
Immunogen affinity purified
The antibody is affinity-purified using the immunogen peptide immobilized on agarose.
多克隆
IgG
Abpromise™承诺保证使用ab10985于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/3000. |
Entrez Gene: 7352 Human
Omim: 602044 Human
SwissProt: P55916 Human
Unigene: 101337 Human
Unigene: 621879 Human
UCP3 is preferentially expressed in muscle
UCP 3 antibody
UCP3 antibody
UCP3_HUMAN antibody
Uncoupling protein 3 antibody
Uncoupling protein 3 mitochondrial proton carrier antibody
Mitochondrial uncoupling protein 3 antibody
SLC25A9 antibody
Solute carrier family 25 member 9 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCP3 antibody (ab10985)
ab10985 (2µg/ml) staining UCP3 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of myocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
