Anti-Drebrin抗体
参阅全部 Drebrin 一抗
兔多克隆抗体to Drebrin
Rabbit
适用于: ICC, WB, IHC-P, ICC/IFmore details
与反应: Mouse, Rat, Chicken, Human
Synthetic peptide conjugated to KLH, corresponding to amino acids 22-42 of Human Drebrin.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
浓度
50 µl 浓度为 5.5 mg/ml
IgG fraction
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
多克隆
IgG
Abpromise™承诺保证使用ab11068于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (1) | Use at an assay dependent concentration. |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 71 kDa. ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. Read More | |
| IHC-P | (4) | Use at an assay dependent concentration. |
| ICC/IF | Use a concentration of 1 µg/ml. |
Entrez Gene: 396496 Chicken
Entrez Gene: 1627 Human
Entrez Gene: 56320 Mouse
Omim: 126660 Human
SwissProt: P18302 Chicken
SwissProt: Q16643 Human
SwissProt: Q9QXS6 Mouse
Unigene: 130316 Human
Unigene: 19016 Mouse
Unigene: 11247 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Drebrin antibody (ab11068)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
Rabbit polyclonal to Drebrin (ab11068) used in IHC(Formalin/PFA-fixed paraffin-embedded sections; Standard stABCpx-DAB detection). ab11068 detecting Drebrin protein on Rat spinal cord E16 embryo sections fixed in formaldehyde. Antigen retrieval step: Heat mediated. 1% BSA as blocking agent for 10 mins at RT. Ab11068 was used at a dilution of 1/2500 incubated for 16 hours. Secondary Antibody is biotin labeled anti rabbit IgG (1/300). Drebrin positivity conformed to published localisation: essentially, many non-neuronal developing tissues were positive, eg: skeletal primary myotubes. This picture is of spinal cord floor plate region (x20). Nuclei were counterstained using Mayer's Haemalum.
Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab11068)This image is a courtesy of Anonymous Abreview
ab11068 staining Drebrin in mouse COR-1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton x100 and blocking with 2% BSA was done at 250C for 15 minutes. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.25% gelatin in PBS) for 3 hours at 25°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab11068)
ICC/IF image of ab11068 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
