Anti-CD33抗体[6C5/2]
参阅全部 CD33 一抗
小鼠单克隆抗体[6C5/2] to CD33
Mouse
适用于: IHC-Pmore details
与反应: Human
Recombinant full length protein (Fc-tag) corresponding to CD33.
IHC-P: Human tonsil FFPE tissue sections
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
浓度
50 µg 浓度为 1 mg/ml
50 µg 浓度为 0.8 mg/ml
Protein A purified
单克隆
6C5/2
Sp2/0-Ag14
IgG1
Abpromise™承诺保证使用ab11032于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (3) | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 945 Human
Omim: 159590 Human
SwissProt: P20138 Human
Unigene: 83731 Human
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (ab11032)](https://www.abcam.cn/ps/products/11/ab11032/Images/ab11032-172917-anti-cd33-antibody-6c5-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD33 antibody [6C5/2] (ab11032)
IHC image of CD33 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11032, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
