Anti-ARF1抗体[ARFS 3F1]
参阅全部 ARF1 一抗
小鼠单克隆抗体[ARFS 3F1] to ARF1
Mouse
This antibody is specific to class I ARF.
适用于: IHC-Pmore details
与反应: Human
Synthetic peptide:
SNQLRNQ
, corresponding to C terminal amino acids 174-180 of Human ARF1.
BALB/3T3 whole cell lysate (ab7901) can be used as a positive control in WB.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
浓度
50 µg 浓度为 1 mg/ml
50 µg 浓度为 0.5 mg/ml
Protein A purified
单克隆
ARFS 3F1
Sp2/0-Ag14
IgG2a
Abpromise™承诺保证使用ab11038于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 375 Human
Omim: 103180 Human
SwissProt: P84077 Human
Unigene: 286221 Human
ARF1 antibody
ARF1_HUMAN antibody
ADP Ribosylation Factor 1 antibody
ADP-ribosylation factor 1 antibody
ARF 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)
IHC image of ab11038 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11038, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.