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Anti-ARF1 antibody [ARFS 3F1]

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  • 产品名称

    Anti-ARF1抗体[ARFS 3F1]
    参阅全部 ARF1 一抗

  • 描述

    小鼠单克隆抗体[ARFS 3F1] to ARF1

  • 宿主

    Mouse

  • 特异性

    This antibody is specific to class I ARF.

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide:

    SNQLRNQ

    , corresponding to C terminal amino acids 174-180 of Human ARF1.

    Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • BALB/3T3 whole cell lysate (ab7901) can be used as a positive control in WB.

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituent: 99.98% PBS

  • 浓度

    • 50 µg 浓度为 1 mg/ml

    • 50 µg 浓度为 0.5 mg/ml

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    ARFS 3F1

  • 骨髓瘤

    Sp2/0-Ag14

  • 同种型

    IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab11038于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
IHC-P

Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 antibody [ARFS 3F1] (ab11038)

IHC image of ab11038 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11038, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.