Anti-DLL1抗体
参阅全部 DLL1 一抗
兔多克隆抗体to DLL1
Rabbit
Activity against Delta-1 from other species has not been determined but is expected based on sequence homology. Expected to cross-react with rat, human and chicken.
适用于: IHC-P, WB, ICC/IF, IHC-Fr, IHC-FoFr, ELISAmore details
与反应: Mouse, Rat, Human
预测可用于: Chicken
Synthetic peptide:
CGEEWSQDLHSSGRTDLRYS
conjugated to KLH, corresponding to amino acids 155-173 of Mouse Delta-1.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
批次浓度范围 100 µg 浓度为 1.05 - 1.21 mg/ml
100 µg 浓度为 1 mg/ml
Affinity purified
Anti-DELTA-1 Antibody was affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
多克隆
IgG
Abpromise™承诺保证使用ab10554于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | Use at an assay dependent concentration. |
| WB | 1/100 - 1/1000. Predicted molecular weight: 78 kDa. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-Fr | (1) | Use at an assay dependent concentration. |
| IHC-FoFr | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
Entrez Gene: 28514 Human
Entrez Gene: 13388 Mouse
Omim: 606582 Human
SwissProt: O00548 Human
SwissProt: Q61483 Mouse
Unigene: 379912 Human
Unigene: 4875 Mouse
Unigene: 10628 Rat
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Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)Greene M et al., PLos One, 2016 Sep 13;11(9):e0161157. Fig 1.; doi: 10.1371/journal.pone.0161157 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
ab10554 staining DLL1 in U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 4% BSA for 1 hour. Samples were incubated with primary antibody for 1 hour at room temperature. DAPI used to stain nuclei
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL1 antibody (ab10554)
ab10554 staining DLL1 in Mouse embryonic kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (2ug/ml) for 1.5 hours at room temperature. A peroxidase-conjugated polyclonal was used as the secondary antibody. Nuclei stained purple with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)
ICC/IF image of ab10554 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10554, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-DLL1 antibody (ab10554)
Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:
Lane 1: Mouse Heart
Lane 2: Mouse Pancreas
Lane 3: Mouse Brain
Lane 4: Rat Heart
Lane 5: Rat Pancreas
Lane 6: Rat Brain
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 1 minute
Expected molecular weight: 77kDa
Cell lysates at 20µg per lane.
NB: This image shows that is was not possible to detect Delta 1 immunoreactivity by WB in the lysates tested. However little is known about Delta 1 expression and it is likely to be developmentally regulated with low expression/abundance making it difficult to detect in whole tissue homogenates. In favour of Delta 1 antibody (ab10554), the staining on the WB shows very low background and little cross reactivity in whole homogenates.
Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:
Lane 1: Mouse Heart
