Anti-KAT13C / NCOA2抗体
参阅全部 KAT13C / NCOA2 一抗
兔多克隆抗体to KAT13C / NCOA2
Rabbit
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
适用于: IP, IHC-P, WBmore details
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide within KAT13C/ NCOA2 aa 1400-1500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q15596
Nuclear or cytosolic extract from HeLa cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab10508于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at 1-4 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 159 kDa. |
Entrez Gene: 10499 Human
Entrez Gene: 17978 Mouse
Omim: 601993 Human
SwissProt: Q15596 Human
SwissProt: Q61026 Mouse
Unigene: 446678 Human
Unigene: 2537 Mouse
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Western blot - Anti-KAT13C / NCOA2 antibody (ab10508)All lanes : Anti-KAT13C / NCOA2 antibody (ab10508) at 1 µg/ml
Lane 1 : TCMK-1 whole cell lysate
Lane 2 : 4T1 whole cell lysate
Lane 3 : CT26.WT whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 159 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13C / NCOA2 antibody (ab10508)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling KAT13C / NCOA2 with ab10508 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-KAT13C / NCOA2 antibody (ab10508)
ab10508 immunoprecipitation of human NCOA2 (SRC2). Samples: Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: ab10508 used at 20 µg/10 mg lysate. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2 (SRC2).
