Anti-GGA1抗体
参阅全部 GGA1 一抗
兔多克隆抗体to GGA1
Rabbit
This affinity purified antibody is directed against human GGA1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Cross reactivity may occur with GGA1 from bovine (87% homology), mouse (82% homology) and rat (76% homology) sources. The immunizing sequence is present only in isoform 1 of this protein, therefore cross reactivity with splice variants is not expected. Cross reactivity with GGA1 homologues from other sources has not been determined.
适用于: WBmore details
与反应: Human
Synthetic peptide:
AMPTRPGEQASPEQPSA
, corresponding to amino acids 336-352 of Human GGA1.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
This affinity purified antibody is directed against human GGA1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Abpromise™承诺保证使用ab10551于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
Entrez Gene: 26088 Human
Omim: 606004 Human
SwissProt: Q9UJY5 Human
Unigene: 499158 Human
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Western blot - Anti-GGA1 antibody (ab10551)
Anti-GGA1 antibody (ab10551) at 1/1350 dilution + Lysate of HEK293 cells overexpressing YFP-GGA1 fusion protein at 35 µg/ml
Secondary
Goat-anti Rabbit IgG
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 100 kDawhy is the actual band size different from the predicted?
The lysate prepared from HEK293 cells overexpressing YFP-GGA1 fusion protein. This accounts for the observed band of 100 kDa.