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Anti-RAD54 antibody [Rad54 4E3/1]

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  • 产品名称

    Anti-RAD54抗体[Rad54 4E3/1]
    参阅全部 RAD54 一抗

  • 描述

    小鼠单克隆抗体[Rad54 4E3/1] to RAD54

  • 宿主

    Mouse

  • 经测试应用

    适用于: IPWBICC/IFFlow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length protein corresponding to Human RAD54.

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituent: 99.98% PBS

  • 浓度

    • 50 µg 浓度为 1.1 mg/ml

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    Rad54 4E3/1

  • 骨髓瘤

    Sp2/0-Ag14

  • 同种型

    IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11055于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
IP(1)

Use at an assay dependent concentration.

WB(2)

Use at an assay dependent concentration.

ICC/IF(1)

Use at an assay dependent concentration.

Flow Cyt

Use 1µg for 106 cells. 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

  • 数据库链接

  • 别名

    • hHR54 antibody

    • HR 54 antibody

    • HR54 antibody

    • hRAD 54 antibody

    • hRAD54 antibody

    • RAD 54A antibody

    • RAD 54L antibody

    • RAD54 (S. cerevisiae) like antibody

    • RAD54 homolog antibody

    • RAD54 like (S. cerevisiae) antibody

    • RAD54 like antibody

    • RAD54 like protein antibody

    • RAD54_HUMAN antibody

    • RAD54A antibody

    • RAD54L antibody

    • DNA repair and recombination protein RAD54 like antibody

    • DNA repair and recombination protein RAD54-like antibody

    • hHR 54 antibody

Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)

Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)

Overlay histogram showing HeLA cells stained with ab11055 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11055, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.