Anti-RAD54抗体[Rad54 4E3/1]
参阅全部 RAD54 一抗
小鼠单克隆抗体[Rad54 4E3/1] to RAD54
Mouse
适用于: IP, WB, ICC/IF, Flow Cytmore details
与反应: Human
Full length protein corresponding to Human RAD54.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
浓度
50 µg 浓度为 1.1 mg/ml
Protein A purified
单克隆
Rad54 4E3/1
Sp2/0-Ag14
IgG1
Abpromise™承诺保证使用ab11055于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP | (1) | Use at an assay dependent concentration. |
WB | (2) | Use at an assay dependent concentration. |
ICC/IF | (1) | Use at an assay dependent concentration. |
Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Entrez Gene: 8438 Human
Omim: 603615 Human
SwissProt: Q92698 Human
Unigene: 642042 Human
hHR54 antibody
HR 54 antibody
HR54 antibody
hRAD 54 antibody
hRAD54 antibody
RAD 54A antibody
RAD 54L antibody
RAD54 (S. cerevisiae) like antibody
RAD54 homolog antibody
RAD54 like (S. cerevisiae) antibody
RAD54 like antibody
RAD54 like protein antibody
RAD54_HUMAN antibody
RAD54A antibody
RAD54L antibody
DNA repair and recombination protein RAD54 like antibody
DNA repair and recombination protein RAD54-like antibody
hHR 54 antibody
Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)
Overlay histogram showing HeLA cells stained with ab11055 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11055, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.