Anti-Cofilin抗体
参阅全部 Cofilin-1 一抗
兔多克隆抗体to Cofilin
Rabbit
适用于: ICC/IFmore details
与反应: Human
Synthetic peptide corresponding to Human Cofilin aa 150-250 conjugated to keyhole limpet haemocyanin (Cysteine residue). Conjugated to maleimide-activated KLH. The immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.
The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
IgG fraction
Whole antiserum is fractionated and further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
多克隆
IgG
Abpromise™承诺保证使用ab11062于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. |
Entrez Gene: 1072 Human
Omim: 601442 Human
SwissProt: P23528 Human
Unigene: 170622 Human
CFL1 antibody
COF1_HUMAN antibody
Cofilin 1 antibody
Cofilin 1 non muscle antibody
Cofilin antibody
Cofilin non muscle isoform antibody
Cofilin-1 antibody
epididymis secretory protein Li 15 antibody
HEL-S-15 antibody
non-muscle isoform antibody
p18 antibody
18 kDa phosphoprotein antibody
CFL 1 antibody
CFL antibody
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (ab11062)
ICC/IF image of ab11062 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11062, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
