Anti-CD41 antibody [M148]

• 产品名称

  Anti-CD41抗体[M148]
  参阅全部 CD41 一抗

• 描述

  小鼠单克隆抗体[M148] to CD41

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• Flow Cyt: HEL92.1.7 cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  M148

• 骨髓瘤

  P3x63-Ag8.653

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab11024于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3674 Human

• Omim: 607759 Human

• SwissProt: P08514 Human

• Unigene: 411312 Human

• 别名

• CD41 antibody

• CD41B antibody

• form 2 antibody

• GP2B antibody

• GPalpha IIb antibody

• GPIIb antibody

• GT antibody

• GTA antibody

• HPA3 antibody

• Integrin alpha 2b antibody

• Integrin alpha IIb antibody

• Integrin alpha-IIb light chain antibody

• Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) antibody

• ITA2B_HUMAN antibody

• Itga2b antibody

• ITGAB antibody

• platelet fibrinogen receptor, alpha subunit antibody

• platelet glycoprotein IIb of IIb/IIIa complex antibody

• Platelet membrane glycoprotein IIb antibody

• platelet specific antigen BAK antibody

• PPP1R93 antibody

• antigen CD41 antibody

• BDPLT16 antibody

• BDPLT2 antibody

• 

  Flow Cytometry - Anti-CD41 antibody [M148] (ab11024)

  Overlay histograms showing left HEL92.1.7 cells and right Jurkat cells stained with ab11024 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab11024) (1x106 in 100µl at 0.1µg/ml) for 30 min on ice.

  The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150177)was used at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable single cells.

• 

  Flow Cytometry - Anti-CD41 antibody [M148] (ab11024)

  Overlay histogram showing HL60 cells stained with ab11024 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11024, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.