Anti-Histone H3 (phospho S28) antibody [HTA28]

• 产品名称

  Anti-Histone H3 (phospho S28)抗体[HTA28]
  参阅全部 Histone H3 一抗

• 描述

  大鼠单克隆抗体[HTA28] to Histone H3 (phospho S28)

• 宿主

  Rat

• 经测试应用

  适用于: WB, ICCmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Hamster, Cow

• 免疫原

  Synthetic peptide corresponding to Human Histone H3 aa 23-35 (phospho S28) conjugated to Keyhole Limpet Haemocyanin (KLH).
  Sequence:

  KAARKSA PATGGV

  
  Database link: P68431

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: HeLa (treated with Nocodazole) whole cell extract. ICC: HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituents: 0.0268% PBS, 1% BSA

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from culture supernatant of hybridoma cells grown in a bioreactor

• 克隆

  单克隆

• 克隆编号

  HTA28

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab10543于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 8350 Human

• Entrez Gene: 8351 Human

• Entrez Gene: 8352 Human

• Entrez Gene: 8353 Human

• Entrez Gene: 8354 Human

• Entrez Gene: 8355 Human

• Entrez Gene: 8356 Human

• Entrez Gene: 8357 Human

• Entrez Gene: 8358 Human

• Entrez Gene: 8968 Human

• Entrez Gene: 319152 Mouse

• Entrez Gene: 319153 Mouse

• Entrez Gene: 360198 Mouse

• Entrez Gene: 97908 Mouse

• Omim: 602810 Human

• SwissProt: P68432 Cow

• SwissProt: P68431 Human

• SwissProt: P68433 Mouse

• Unigene: 132854 Human

• Unigene: 247813 Human

• Unigene: 247814 Human

• Unigene: 248176 Human

• Unigene: 443021 Human

• Unigene: 484990 Human

• Unigene: 532144 Human

• Unigene: 533292 Human

• Unigene: 546315 Human

• Unigene: 586261 Human

• Unigene: 591778 Human

• Unigene: 221301 Mouse

• Unigene: 261657 Mouse

• Unigene: 377874 Mouse

• Unigene: 390558 Mouse

• Unigene: 397328 Mouse

别名

• H3 histone family member E pseudogene antibody

• H3 histone family, member A antibody

• H3/A antibody

• H31_HUMAN antibody

• H3F3 antibody

• H3FA antibody

• Hist1h3a antibody

• HIST1H3B antibody

• HIST1H3C antibody

• HIST1H3D antibody

• HIST1H3E antibody

• HIST1H3F antibody

• HIST1H3G antibody

• HIST1H3H antibody

• HIST1H3I antibody

• HIST1H3J antibody

• HIST3H3 antibody

• histone 1, H3a antibody

• Histone cluster 1, H3a antibody

• Histone H3 3 pseudogene antibody

• Histone H3.1 antibody

• Histone H3/a antibody

• Histone H3/b antibody

• Histone H3/c antibody

• Histone H3/d antibody

• Histone H3/f antibody

• Histone H3/h antibody

• Histone H3/i antibody

• Histone H3/j antibody

• Histone H3/k antibody

• Histone H3/l antibody

• 

  Western blot - Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543)

  Lane 1 : Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543) at 0.5 µg/ml
  Lane 2 : Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543) at 1 µg/ml
  Lane 3 : Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543) at 2 µg/ml
  
  All lanes : HeLa (treated with Nocodazole) whole cell extract
  
  Secondary
  All lanes : Goat Anti-Rat IgG-peroxidase conjugate
  
  Developed using the ECL technique.
  

• 

  Immunocytochemistry - Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543)

  Immunocytochemical analysis of HeLa cells fixed and permeabilized with methanol followed by methanol:acetone. Histone H3 (phospho S28) was labeled with ab10543 at 5 μg/mL in ICC/IF (Green). The secondary antobody was a Goat Anti-Rat IgG-FITC conjugate. The nuclear counerstain was DAPI (Blue).

• 

  Immunocytochemistry - Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543)This image is courtesy of an anonymous Abreview

  ab10543 staining Histone H3 (phospho S28) in Human neural progenitor cells from iPS cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 1% serum, 0.1% BSA in PBS for 30 minutes at room temperature. Samples were incubated with primary antibody (2ug/ml in blocking buffer) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rat IgG2a polyclonal was used as the secondary antibody (1/500). Total cells were stained using DAPI (blue)

  See Abreview

• 

  Western blot - Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543)

  All lanes : Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543) at 1 µg/ml
  
  Lane 1 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated
  Lane 2 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated with Human Histone H3 (unmodified ) peptide (ab2623) at 0.5 µg/ml
  Lane 3 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated with Human Histone H3 (phospho S28) peptide (ab5499) at 0.5 µg/ml
  Lane 4 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated with Human Histone H3 (phospho S10) peptide (ab11477) at 0.5 µg/ml
  
  Lysates/proteins at 2.5 µg per lane.
  
  Secondary
  All lanes : Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Observed band size: 17 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 20 minutes
  
  
  

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab10543 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

• 

  Immunocytochemistry - Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543)This image is courtesy of an anonymous Abreview

  ab10543 staining Histone H3 (phospho S28) in Mouse neural stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 4% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 4% serum) for 16 hours at 4°C. An Alexa Fluor® 546-conjugated anti-rat polyclonal was used as the secondary antibody (1/500). DAPI is stainded blue

  See Abreview

• 

  Immunocytochemistry - Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543)This image is courtesy of an Abreview submitted by Dr Kirk McManus

  ab10543 staining HeLa cells by ICC/IF. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X100 in PBS. The cells were then stained with ab10543 at 1/1000 in PBS for 1h at 22°C. A goat anti-rat Alexa Fluro 488 (ab150157) at 1/200 was used as the secondary antibody. Nuclei are stained in red with DAPI. The antibody produces the expected mitotic-associated staining pattern and is extremely strong. MeOH fixed samples were also evaluated and produced a similar, strong staining pattern in mitotic cells.