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| Product Name | Ring1 [F4-C12] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Ring1A antibody E3 ubiquitin-protein ligase RING1 antibody Polycomb complex protein RING1 antibody Really interesting new gene 1 protein antibody RING finger protein 1 antibody Ring1 antibody RING1_HUMAN antibody RING1A antibody Rnf1 antibody Transcription repressor Ring1A antibody |
| Product description | In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci during embryonic development. The human PcG homologues form two distinct multimeric protein complexes, the EED/EZH protein complex and the HPC/HPH protein complex, which have mutually exclusive expression patterns. The HPC/HPH PcG complex contains the human polycomb 2 (HPC2), human polyhomeotic (HPH), Bmi-1 and RING1 proteins. The human RING1 gene, which is proximal to the major histocompatibility complex region on chromosome six, encodes for a protein that contains a RING finger motif, a zinc-binding domain found in many regulatory proteins, but unlike the other human PcG genes, RING1 displays no homology to known Drosophila PcG genes. RING1 strongly represses En-2, the mammalian homolog of the Drosophila engrailed gene, and when overexpressed, it mediates an increase in the expression of proto-oncogenes, such as c-Jun and c-fos. Also, loss of RING1 and Bmi-1 expression correlates with the differentiation of B cells, which suggests a role for RING1 in germinal center development. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:100-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Ring1A antibody E3 ubiquitin-protein ligase RING1 antibody Polycomb complex protein RING1 antibody Really interesting new gene 1 protein antibody RING finger protein 1 antibody Ring1 antibody RING1_HUMAN antibody RING1A antibody Rnf1 antibody Transcription repressor Ring1A antibody |
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| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Nucleus speckle. |
| SwissProt ID | Q06587 |
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Application
Fig1: Western blot analysis of Ring1 on human Ring1 recombinant protein using anti-Ring1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of Ring1 on HEK293 (1) and Ring1-hIgGFc transfected HEK293 (2) cell lysate using anti-Ring1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of Ring1 on different cell lysate using anti-Ring1 antibody at 1/1,000 dilution. Positive control： Line1: MOLT-4? Line2: LNCaP Line3: Hela Line4: HEK-293? Line5: Jurkat
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with Ring1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human Ring1 recombinant protein, Ring1-hIgGFc transfected HEK293 cell lysate, human cervical cancer tissue, human rectum cancer tissue, MOLT-4?, LNCaP, Hela, HEK-293?, Jurkat cell lysate. Hela. |
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| Application Notes | WB:1:500-1:1,000 IHC:1:100-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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