| 存储条件 |
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| Product Name | ERN1 [C9-F2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Endoplasmic reticulum (ER) to nucleus signalling 1 antibody Endoplasmic reticulum to nucleus signaling 1 antibody Endoplasmic reticulum-to-nucleus signaling 1 antibody Endoribonuclease antibody ER to nucleus signaling 1 antibody ERN 1 antibody Ern1 antibody ERN1_HUMAN antibody hIRE 1p antibody hIRE1p antibody Inositol requiring 1 antibody Inositol requiring 1, S. cerevisiae, homolog of antibody Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody Inositol requiring protein 1 antibody inositol-requiring enzyme 1 antibody Inositol-requiring protein 1 antibody IRE 1 antibody IRE 1a antibody IRE 1P antibody Ire1 alpha antibody Ire1-alpha antibody IRE1a antibody Ire1alpha antibody IRE1P antibody MGC163277 antibody MGC163279 antibody Protein kinase/endoribonuclease antibody RGD1559716 antibody Serine/threonine protein kinase/endoribonuclease IRE1 antibody |
| Product description | The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes. In response to stress, sensors for the ER mammalian cells activate IRE1α and IRE1β, which then results in the phosphorylation of JNK (Jun N-Terminal Kinase) and the activation of the cellular MAP kinase pathway. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Endoplasmic reticulum (ER) to nucleus signalling 1 antibody Endoplasmic reticulum to nucleus signaling 1 antibody Endoplasmic reticulum-to-nucleus signaling 1 antibody Endoribonuclease antibody ER to nucleus signaling 1 antibody ERN 1 antibody Ern1 antibody ERN1_HUMAN antibody hIRE 1p antibody hIRE1p antibody Inositol requiring 1 antibody Inositol requiring 1 S. cerevisiae homolog of antibody Inositol requiring enzyme 1 S. cerevisiae homolog of antibody Inositol requiring protein 1 antibody inositol-requiring enzyme 1 antibody Inositol-requiring protein 1 antibody IRE 1 antibody IRE 1a antibody IRE 1P antibody Ire1 alpha antibody Ire1-alpha antibody IRE1a antibody Ire1alpha antibody IRE1P antibody MGC163277 antibody MGC163279 antibody Protein kinase/endoribonuclease antibody RGD1559716 antibody Serine/threonine protein kinase/endoribonuclease IRE1 antibody |
|---|---|
| Cellular Localization | Endoplasmic reticulum membrane. |
| SwissProt ID | O75460 |
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Application
Fig1: Western blot analysis of ERN1 on different cell lysate using anti-ERN1 antibody at 1/1,000 dilution. Positive control： Line1: Raji Line2: A431 Line3: Jurkat Line4: Hela Line5: HEK293
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERN1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ERN1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-ERN1 antibody. Counter stained with hematoxylin.| Positive Control | Raji, A431, Jurkat, Hela, HEK293 cell lysate, human placenta tissue, human brain tissue, human stomach tissue. |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
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