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| Product Name | ABCC4 [H3-A7] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | ABCC 4 antibody ABCC4 antibody ATP binding cassette sub family C (CFTR/MRP) member 4 antibody ATP binding cassette sub family C member 4 antibody ATP-binding cassette sub-family C member 4 antibody bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody bA464I2.1 antibody Canalicular multispecific organic anion transporter antibody Canalicular multispecific organic anion transporter ABC superfamily antibody EST170205 antibody MOAT B antibody MOAT-B antibody MOATB antibody MRP 4 antibody MRP/cMOAT related ABC transporter antibody MRP/cMOAT-related ABC transporter antibody MRP4_HUMAN antibody Multi specific organic anion transporter B antibody Multi-specific organic anion transporter B antibody Multidrug resistance associated protein 4 antibody Multidrug resistance-associated protein 4 antibody OTTHUMP00000018560 antibody |
| Product description | The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the Mdr-1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an MDR-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter), and MRP3, which are both conjugate export pumps expressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the basolateral membrane where it also mediates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired. MRP6 is highly expressed in liver and kidney, whereas MRP4 and MRP5 are detected in various tissues, yet at much lower levels of expression. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:100-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ABCC 4 antibody ABCC4 antibody ATP binding cassette sub family C (CFTR/MRP) member 4 antibody ATP binding cassette sub family C member 4 antibody ATP-binding cassette sub-family C member 4 antibody bA464I2.1 (ATP binding cassette sub-family C (CFTR/MRP) member 4) antibody bA464I2.1 antibody Canalicular multispecific organic anion transporter antibody Canalicular multispecific organic anion transporter ABC superfamily antibody EST170205 antibody MOAT B antibody MOAT-B antibody MOATB antibody MRP 4 antibody MRP/cMOAT related ABC transporter antibody MRP/cMOAT-related ABC transporter antibody MRP4_HUMAN antibody Multi specific organic anion transporter B antibody Multi-specific organic anion transporter B antibody Multidrug resistance associated protein 4 antibody Multidrug resistance-associated protein 4 antibody OTTHUMP00000018560 antibody |
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| Molecular Weight(MW) | 150 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | O15439 |
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Application
Fig1: Western blot analysis of ABCC4 on human ABCC4 recombinant protein using anti-ABCC4 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ABCC4 on HEK293 (1) and ABCC4-hIgGFc transfected HEK293 (2) cell lysate using anti-ABCC4 antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABCC4 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-ABCC4 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of A549 cells with ABCC4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red)| Positive Control | Human ABCC4 recombinant protein, ABCC4-hIgGFc transfected HEK293 cell lysate, human liver cancer tissue, human esophagus cancer tissue, A549. |
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| Application Notes | WB:1:500-1:1,000 IHC:1:100-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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