| 存储条件 |
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| Product Name | GUCY1A3 [B2-G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GC S alpha 1 antibody GC SA3 antibody GCS alpha 1 antibody GCS alpha 3 antibody GCS-alpha-1 antibody GCS-alpha-3 antibody GCYA3_HUMAN antibody Guanylate cyclase 1 soluble alpha 3 antibody Guanylate cyclase soluble subunit alpha 1 antibody Guanylate cyclase soluble subunit alpha 3 antibody Guanylate cyclase soluble subunit alpha-3 antibody Guanylate cyclase, nitric oxide-sensitive, alpha-1 subunit antibody Guc1a1 antibody GUC1A3 antibody GUCA3 antibody GUCSA3 antibody Gucy1a1 antibody Gucy1a3 antibody MYMY6 antibody nitric oxide-sensitive Guanylate cyclase, alpha-1 subunit antibody NOGC, alpha-1 subunit antibody Soluble guanylate cyclase large subunit antibody |
| Product description | Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble forms, known as GCS or sGC, act as receptors for nitric oxide. The membrane-bound receptor forms, known as GC, are peptide hormone receptors. GCS, a cGMP-synthesizing enzyme, is the major receptor for the neurotransmitter nitric oxide (NO). It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme containing heterodimer, consisting of one α subunit, designated GCS-α-1, and one β subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide, which weakly stimulates the enzyme. Both NO and CO stimulation are enhanced by the allosteric activator 3-(5'-hydroxymethyl-2'furyl)-benzyl -indazole, YC-1. YC-1 can also stimulate GCS in a NO-independent manner. Both the α and β subunits are required for cGMP generation, and at least two isoforms exist for each subunit. Heterodimers consisting of α-1/β-1 and α-2/β-1 have been identified, and both display similar enzymatic activity. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | GC S alpha 1 antibody GC SA3 antibody GCS alpha 1 antibody GCS alpha 3 antibody GCS-alpha-1 antibody GCS-alpha-3 antibody GCYA3_HUMAN antibody Guanylate cyclase 1 soluble alpha 3 antibody Guanylate cyclase soluble subunit alpha 1 antibody Guanylate cyclase soluble subunit alpha 3 antibody Guanylate cyclase soluble subunit alpha-3 antibody Guanylate cyclase nitric oxide-sensitive alpha-1 subunit antibody Guc1a1 antibody GUC1A3 antibody GUCA3 antibody GUCSA3 antibody Gucy1a1 antibody Gucy1a3 antibody MYMY6 antibody nitric oxide-sensitive Guanylate cyclase alpha-1 subunit antibody NOGC alpha-1 subunit antibody Soluble guanylate cyclase large subunit antibody |
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| Molecular Weight(MW) | 77 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q02108 |
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Application
Fig1: Western blot analysis of GUCY1A3 on HEK293 (1) and GUCY1A3-hIgGFc transfected HEK293 (2) cell lysate using anti-GUCY1A3 antibody at 1/1,000
Application
Fig2: Western blot analysis of GUCY1A3 on different cell lysate using anti-GUCY1A3 antibody at 1/1,000 dilution. Positive control： Line1:HEK293 Line2:Raji
Application
Fig3: ICC staining GUCY1A3 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using anti-GUCY1A3 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-GUCY1A3 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HEK293 cells with GUCY1A3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).| Positive Control | GUCY1A3-hIgGFc transfected HEK293 cell lysate, HEK293, Raji cell lysate, HepG2, human renal cancer tissue, human esophageal cancer tissue, HEK293. |
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| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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