| 存储条件 |
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| Product Name | TACC3 [SN73-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERIC 1 antibody ERIC-1 antibody ERIC1 antibody MGC117382 antibody MGC133242 antibody OTTHUMP00000113796 antibody TACC3 antibody TACC3_HUMAN antibody Transforming acidic coiled coil containing protein 3 antibody Transforming acidic coiled-coil-containing protein 3 antibody |
| Product description | TACC1 (transforming acidic coiled coil gene 1) is one of three TACC family members, which are thought to be involved in breast tumorigenesis. TACC1 is located on 8p11 chromosomal region that is amplified in approximately 15% of all breast tumor samples. The short arm of chromosome 8 also contains FGFR1 whose expression is enhanced in most breast cancer tumors. TACC family members, TACC1, TACC2, and TACC3, map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. Also, TACC1 contains a conserved C-terminal region as in the Drosophila homolog, D-TACC. It has been shown that D-TACC is necessary for normal spindle function, and the mammalian TACC proteins appears to interact with centrosomes and microtubules in a similar manner. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:100-1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | ERIC 1 antibody ERIC-1 antibody ERIC1 antibody MGC117382 antibody MGC133242 antibody OTTHUMP00000113796 antibody TACC3 antibody TACC3_HUMAN antibody Transforming acidic coiled coil containing protein 3 antibody Transforming acidic coiled-coil-containing protein 3 antibody |
|---|---|
| Molecular Weight(MW) | 140 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of TACC3 on Hela cells lysates using anti-TACC3 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining TACC3 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TACC3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining TACC3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TACC3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TACC3 antibody. Counter stained with hematoxylin.| Positive Control | PANC-1, PMVEC, Hela, PC-3M, mouse testis tissue, human tonsil tissue. |
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| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:100-1:500 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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