| 存储条件 |
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| Product Name | NR1I2 [G1-D12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BXR antibody Nr1i2 antibody NR1I2_HUMAN antibody Nuclear receptor subfamily 1 group I member 2 antibody ONR 1 antibody ONR1 antibody Orphan nuclear receptor PAR 1 antibody Orphan nuclear receptor PAR1 antibody Orphan nuclear receptor PXR antibody OTTHUMP00000215173 antibody OTTHUMP00000215174 antibody OTTHUMP00000215175 antibody PAR 1 antibody PAR 2 antibody PAR antibody PAR q antibody PAR1 antibody PAR2 antibody PARq antibody pregnane X nuclear receptor variant 2 antibody Pregnane X receptor antibody PRR antibody PXR antibody SAR antibody Steroid and xenobiotic receptor antibody SXR antibody |
| Product description | Steroid hormones function as signaling molecules by diffusing into cells and interacting with specific intracellular receptors to regulate gene expression. This superfamily of receptors includes both steroid and nonsteroid receptors. Like many nonsteroid hormone receptors, PXR (Pregnane X Receptor) binds as a heterodimer with RXR to a DNA sequence typical of a nonsteroid hormone receptor; however, PXR is activated by several steroids, such as naturally occurring pregnanes and synthetic glucocorticoids and anti- glucocorticoids. PXR exists as two alternatively spliced isoforms, PXR.1 and PXR.2. PXR is thought to define a novel steroid hormone signaling pathway that may account for some of the effects of synthetic glucocorticoids and antiglucocorticoids that are not mediated through the classical glucocorticoid receptor signaling pathway. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | BXR antibody Nr1i2 antibody NR1I2_HUMAN antibody Nuclear receptor subfamily 1 group I member 2 antibody ONR 1 antibody ONR1 antibody Orphan nuclear receptor PAR 1 antibody Orphan nuclear receptor PAR1 antibody Orphan nuclear receptor PXR antibody OTTHUMP00000215173 antibody OTTHUMP00000215174 antibody OTTHUMP00000215175 antibody PAR 1 antibody PAR 2 antibody PAR antibody PAR q antibody PAR1 antibody PAR2 antibody PARq antibody pregnane X nuclear receptor variant 2 antibody Pregnane X receptor antibody PRR antibody PXR antibody SAR antibody Steroid and xenobiotic receptor antibody SXR antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Nucleus,intermediate filament cytoskeleton. |
| SwissProt ID | O75469 |
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Application
Fig1: Western blot analysis of NR1I2 on human NR1I2 recombinant protein using anti-NR1I2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of NR1I2 on HEK293 (1) and NR1I2-hIgGFc transfected HEK293 (2) cell lysate using anti-NR1I2 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining NR1I2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of HepG2 cells with NR1I2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human NR1I2 recombinant protein, NR1I2-hIgGFc transfected HEK293 cell lysate, Hela, HepG2. |
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| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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