| 存储条件 |
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| Product Name | JARID2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JARD2 antibody JARD2_HUMAN antibody JARID2 antibody JMJ antibody Jumonji AT rich interactive domain 2 antibody Jumonji homolog antibody Jumonji like protein antibody Jumonji protein antibody Jumonji/ARID domain containing protein 2 antibody Jumonji/ARID domain-containing protein 2 antibody Protein Jumonji antibody |
| Product description | Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | JARD2 antibody JARD2_HUMAN antibody JARID2 antibody JMJ antibody Jumonji AT rich interactive domain 2 antibody Jumonji homolog antibody Jumonji like protein antibody Jumonji protein antibody Jumonji/ARID domain containing protein 2 antibody Jumonji/ARID domain-containing protein 2 antibody Protein Jumonji antibody |
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| Molecular Weight(MW) | 139 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q92833 |
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Application
Fig1: Western blot analysis of JARID2 on human JARID2 recombinant protein using anti-JARID2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of JARID2 on HEK293 (1) and JARID2-hIgGFc transfected HEK293 (2) cell lysate using anti-JARID2 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining JARID2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of Hela cells with JARID2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human JARID2 recombinant protein, JARID2-hIgGFc transfected HEK293 cell lysate, Hela. |
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| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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