Anti-SMC3抗体
参阅全部 SMC3 一抗
兔多克隆抗体to SMC3
Rabbit
适用于: IP, WB, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to Human SMC3 conjugated to keyhole limpet haemocyanin. Which represents a portion within the last 100 amino acids GenBank PID 4885399.
WB: HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH/3T3 whole cell lysate; IHC-P: Human breast carcinoma and mouse renal cell carcinoma; IP: Lysate from HEK293T cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
多克隆
IgG
Abpromise™承诺保证使用ab9263于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (1) | Use at 2-10 µg/mg of lysate. |
| WB | (3) | 1/10000 - 1/25000. Detects a band of approximately 140 kDa (predicted molecular weight: 141.5 kDa). |
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 395188 Chicken
Entrez Gene: 9126 Human
Entrez Gene: 13006 Mouse
Omim: 606062 Human
SwissProt: Q9UQE7 Human
SwissProt: Q9CW03 Mouse
SwissProt: Q5R4K5 Orangutan
Unigene: 24485 Human
Unigene: 14910 Mouse
Unigene: 11074 Rat
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Western blot - Anti-SMC3 antibody (ab9263)
All lanes : Anti-SMC3 antibody (ab9263) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : LNCaP (human prostate cancer cell line) whole cell lysate
Lane 5 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 7 : TCMK-1 (mouse epithelial cell line from kidney transformed with SV40 viral antigen) whole cell lysate
Lane 8 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 141.5 kDa
Exposure time: 3 seconds
Lysates prepared using NETN lysis buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (ab9263)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling SMC3 with ab9263 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (ab9263)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labeling SMC3 with ab9263 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.
Immunoprecipitation - Anti-SMC3 antibody (ab9263)
Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.
Lane 1: ab9263 used for IP at 6 µg per reaction
Lane 2: Control IgG
For blotting immunoprecipitated SMC3, ab9263 was used at 0.04 µg/mL.
Detection: Chemiluminescence with an exposure time of 3 seconds.
