Anti-V5 tag antibody

• 产品名称

  Anti-V5 tag抗体
  参阅全部 V5 tag 一抗

• 描述

  兔多克隆抗体to V5 tag

• 宿主

  Rabbit

• 特异性

  ELISA: V5 tag antibody - ChIP Grade (ab9116) specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.

• 经测试应用

  适用于: WB, ELISA, IP, IHC-Pmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Synthetic peptide corresponding to Parainfluenza virus 5 V5 tag aa 50-150 conjugated to keyhole limpet haemocyanin. (V5 epitope)
  Database link: P11208

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 纯度

  Immunogen affinity purified

• 纯化说明

  Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg .

• 克隆

  多克隆

• 同种型

  IgG

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9116于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

别名

• GKPIPNPLLGLDST epitope tag antibody

• GKPIPNPLLGLDST tag antibody

• Protein Rev antibody

• Regulator of expression of viral proteins antibody

• rev antibody

• V5 epitope tag antibody

• 

  Western blot - Anti-V5 tag antibody (ab9116)This image is courtesy of an anonymous Abreview

  All lanes : Anti-V5 tag antibody (ab9116) at 1/5000 dilution
  
  Lanes 1-3 : Cells transfected with v5-tagged protein.
  Lanes 4-5 : Untransfected cells.
  
  Secondary
  Lanes 1-3 : Anti-rabbit Ig-HRP at 1/20000 dilution
  Lanes 4-5 : Anti-rabbit Ig-HRP at 1/20000 dilution
  
  
  Mouse ES cells lysate.

  See Abreview