Anti-Myc tag抗体
参阅全部 Myc tag 一抗
兔多克隆抗体to Myc tag
Rabbit
ELISA: Antibody specificity was verified by ELISA against the peptide (EQKLISEEDL). A 1:60,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP-conjugated Goat Anti Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run.
适用于: IHC-Fr, IP, WB, IHC-P, ICC/IF, Electron Microscopymore details
与反应: Species independent
Synthetic peptide corresponding to Myc tag conjugated to keyhole limpet haemocyanin.
ICC/IF: CHO cells transfected with 12-tags constructs. IHC-P: Mouse brain tissue stained for Myc tag. EM: Mouse embryonic fibroblasts expressing myc-tagged protein. IP: Huh7 expressing myc-tagged CDC42 protein and transfected 293FT cells. WB: Y. enterocolitica ?HOPEMT carrying plasmids encoding Myc-tagged CT082 or CT695, and HA-tagged Slc1.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium azide
Affinity purified
Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg of IgG.
多克隆
IgG
Abpromise™承诺保证使用ab9106于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (1) | 1/500. |
| IP | (9) | Use at an assay dependent concentration. |
| WB | (14) | Use at an assay dependent concentration. |
| IHC-P | (3) | Use at an assay dependent concentration. |
| ICC/IF | (10) | 1/1000. |
| Electron Microscopy | Use at an assay dependent concentration. See Abreview for further details (submitted by Eeva-Liisa Eskelinen). |
Nuclear
c-myc tag antibody
Myc Epitope Tag antibody
Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (ab9106)
Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilized with 0.1% Triton X-100 in CHO cells transfected with 12-tags constructs (named as CHO-12 tags, Top) and parental CHO cells (Bottom) labelling MYC with ab9106 at 1/1000 dilution (1 μg/mL), followed by Donkey Anti-rabbit IgG (H&L), FITC conjugated antibody at 1/1000 dilution (1 μg/mL) (Green). Nucleus was counterstained with DAPI (Blue).
Western blot - Anti-Myc tag antibody (ab9106)Pais et al PLoS One. 2013;8(2):e56292. doi: 10.1371/journal.pone.0056292. Epub 2013 Feb 19. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Y. enterocolitica ΔHOPEMT carrying plasmids encoding Myc-tagged CT082 or CT695, and HA-tagged Slc1 (as indicated) were grown in non-secreting conditions. Chloramphenicol was added (time?=?0 min) to stop bacterial protein synthesis and samples were taken at the indicated time points. Samples were analyzed by immunoblotting using ab9106, anti-HA, and anti-TEM antibodies.
Immunoprecipitation - Anti-Myc tag antibody (ab9106)This image is courtesy of an anonymous abreview.
ab9106 at 4 µg/mg lysate used in Huh7 (Human cell line) whole cell lysate (1x106 cells).
Cells expressed myc-tagged CDC42 protein. Immunoprecipitation step performed using Protein A matrix. Incubation time 4 hours at 4°C. Western Blot antibody used at 1/300 dilution.
Immunoprecipitation - Anti-Myc tag antibody (ab9106)
ab9106 used at 1/250 in the immunoprecipitation of transfected human 293FT cells (whole cell lysate).
In this experiment cells were co-transfected with myc tagged cdc25 along with an HA tagged viral protein which binds to cdc25. The cell lysate was immunoprecipitated using ab9106, run on a 10% gel. The blot was probed for HA and HA tagged protein (which co-IPed with Myc tagged protein) and this is seen at ~15KDa.
Lane 1: Input non-transfected cells
Lane 2: Immunoprecipitated non-transfected cells
Lane 3: Input co-transfected cells
Lane 4: Immunoprecipitated co-transfected cells
This image is courtesy of an Abreview submitted on 23 March 2006.
Immunohistochemistry (Frozen sections) - Anti-Myc tag antibody (ab9106)This image is courtesy of an anonymous abreview.
Paraformaldehyde-fixed frozen mouse brain tissue stained for Myc tag using ab9106 at a 1/500 dilution (2hrs at 25°C), followed by a goat anti-rabbit Alexa-Fluor® 568 secondary used at a 1/500 dilution.
For further details please see abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myc tag antibody (ab9106)This image is courtesy of Karen Lee by Abreview.
Paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized mouse brain tissue stained for Myc tag using ab9106 at a 1/500 dilution (15 hrs, 4°C) followed by and Alexa-Fluor® 555 donkey anti-rabbit secondary used at a 1/500 dilution.
For further details please see abreview.
Electron Microscopy - Anti-Myc tag antibody (ab9106)This image is courtesy of an Abreview submitted by Dr Eeva-Liisa Eskelinen
ab9106 at 1/2000 staining mouse embryonic fibroblasts expressing myc-tagged protein by ICC.
The cells were paraformaldehyde fixed and blocked with 3% BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit IgG antibody (10 nm gold conjugated) was used as the secondary.
The image shown is immuno electron microscopical staining with thawed cryosections.
