Anti-Myelin PLP antibody [plpc 1]

• 产品名称

  Anti-Myelin PLP抗体[plpc 1]
  参阅全部 Myelin PLP 一抗

• 描述

  小鼠单克隆抗体[plpc 1] to Myelin PLP

• 宿主

  Mouse

• 特异性

  This antibody recognizes myelin proteolipid protein

• 经测试应用

  适用于: WB, IHC-Fr, IHC-P, ICC/IFmore details
  不适用于: Flow Cyt (Intra)

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Synthetic peptide:

  C-GRGTKF

  , corresponding to amino acids 272-277 of Myelin PLP.

  Run BLAST with Run BLAST with 

• 常规说明

  This product was switched from a hybridoma to a recombinant production format on 27th October 2021.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 浓度

• 100 µg 浓度为 0.809 mg/ml

• 1 mg 浓度为 0.809 mg/ml

• 40 µg 浓度为 0.809 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  plpc 1

• 骨髓瘤

  Sp2/0

• 同种型

  IgG2a

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9311于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for Flow Cyt (Intra).

数据库链接

• Entrez Gene: 5354 Human

• Entrez Gene: 18823 Mouse

• Entrez Gene: 24943 Rat

• Omim: 300401 Human

• SwissProt: P60201 Human

• SwissProt: P60202 Mouse

• SwissProt: P60203 Rat

• Unigene: 1787 Human

• Unigene: 1268 Mouse

• Unigene: 4550 Rat

别名

• HLD1 antibody

• Lipophilin antibody

• Major myelin proteolipid protein antibody

• MMPL antibody

• Myelin proteolipid protein antibody

• MYPR_HUMAN antibody

• PLP 1 antibody

• PLP antibody

• PLP/DM20 antibody

• PLP1 antibody

• PMD antibody

• Proteolipid protein 1 antibody

• SPG2 antibody

• 

  Western blot - Anti-Myelin PLP antibody [plpc 1] (ab9311)

  All lanes : Anti-Myelin PLP antibody [plpc 1] (ab9311) at 1/1000 dilution
  
  Lane 1 : Human cerebellum tissue lysate at 20 µg
  Lane 2 : Human liver tissue lysate at 40 µg
  
  Secondary
  All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
  
  Predicted band size: 30 kDa
  Observed band size: 20.23 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 3 minutes
  
  
  

  Samples are non-boiled as boiling may cause protein aggregates.

  The molecular weight observed is consistent with what has been described in the literature (PMID: 9247276).

  Negative control: liver (PMID: 2414013).

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [plpc 1] (ab9311)

  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

  The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [plpc 1] (ab9311)

  Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

  The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [plpc 1] (ab9311)

  Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

  The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• 

  Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [plpc 1] (ab9311)

  Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [plpc 1] (ab9311)

  Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [plpc 1] (ab9311)

  Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [plpc 1] (ab9311)

  Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [plpc 1] (ab9311)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Myelin PLP with ab9311 at 1/25 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1:1000 dilution (Green). is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.