Anti-6X His tag®抗体
参阅全部 6X His tag® 一抗
鸡多克隆抗体to 6X His tag®
Chicken
Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run.
适用于: WB, ELISAmore details
与反应: Species independent
Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Preservative: 0.1% Sodium azide
浓度
250 µg 浓度为 1 mg/ml
IgY fraction
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
多克隆
unknown
unknown
Abpromise™承诺保证使用ab9107于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use at an assay dependent concentration. Predicted molecular weight: 1 kDa. This antibody was an effective at 1:1000 (1 µg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding. Read More | |
ELISA | Use at an assay dependent concentration. |
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