Anti-CD34 antibody [MEC 14.7]

• 产品名称

  Anti-CD34抗体[MEC 14.7]
  参阅全部 CD34 一抗

• 描述

  大鼠单克隆抗体[MEC 14.7] to CD34

• 宿主

  Rat

• 特异性

  Recognizes mouse CD34.

• 经测试应用

  适用于: IHC-P, WBmore details

• 种属反应性

  与反应: Mouse
  

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• IHC-P: Mouse lung and brain tissue. WB: Mouse lung membrane tissue lysate.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  单克隆

• 克隆编号

  MEC 14.7

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab8158于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 12490 Mouse

• SwissProt: Q64314 Mouse

• Unigene: 29798 Mouse

• 别名

• CD34_HUMAN antibody

• Cluster designation 34 antibody

• cluster of differentiation 34 antibody

• Hematopoietic progenitor cell antigen CD34 antibody

• HPCA1 antibody

• Mucosialin antibody

• OTTHUMP00000034733 antibody

• OTTHUMP00000034734 antibody

• CD34 antibody

• CD34 antigen antibody

• CD34 molecule antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)

  IHC image of CD34 staining in a formalin fixed paraffin embedded mouse lung tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8158, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• 

  Western blot - Anti-CD34 antibody [MEC 14.7] (ab8158)

  Anti-CD34 antibody [MEC 14.7] (ab8158) at 1 µg/ml + Mouse Lung Membrane Tissue Lysate (ab171830) at 50 µg
  
  Secondary
  Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/20000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 41 kDa
  Observed band size: 80 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 2 minutes
  
  
  

  The main band is as expected at 80 kDa since the target is heavily glycosylated and phosphorylated.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)

  IHC image of CD34 staining in a formalin fixed paraffin embedded mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8158, 10 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)This image is courtesy of an anonymous Abreview

  Immunohistochemical analysis of murine lung tissue, staining CD34 with ab8158.
  
  Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 16 hours at 4°C. An undiluted HRP-conjugated horse anti-rat polyclonal IgG was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)This image is courtesy of an anonymous Abreview.

  ab8158 staining CD34 in mouse mammary gland tissue sections by Immunohistochemistry (IHC-P - paraffin-embedded sections). Tissue was fixed with methacarnoy and blocked with 4% BSA for 60 minutes; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) for 16 hours. A undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] (ab8158)This image is courtesy of an anonymous Abreview

  ab8158 staining mouse brain tissue sections by IHC-P.  Tissue sections were paraformaldehyde fixed and blocked with 0.5% Perkin-Elmer TNB Blocking Buffer for 30 minutes at 25°C.  The primary antibody was diluted 1/100 and incubated for 18 hours at 4°C.  An biotin conjugated goat anti-rat was used as the secondary

  Photomicrograph demonstrates CD34 in red and collagen type IV (ab19808) in blue in normal, adult brain vessels. Tissue was perfusion-fixed and cut into 15µm slide-mounted cryostat sections (i.e., lightly fixed, but not paraffin embedded).

  See Abreview