Anti-M6PR (cation independent) antibody [MEM-238]

• 产品名称

  Anti-M6PR (cation independent)抗体[MEM-238]
  参阅全部 M6PR (cation independent) 一抗

• 描述

  小鼠单克隆抗体[MEM-238] to M6PR (cation independent)

• 宿主

  Mouse

• 特异性

  CD222 antigen (human)

• 经测试应用

  适用于: WB, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  预测可用于: Non human primates

• 免疫原

  Tissue, cells or virus corresponding to Human M6PR (cation independent).
  Database link: P11717

• 表位

  Recognizes an epitope between domains 2 and 5.

• 阳性对照

• Flow Cyt (Intra): HeLa cells.

• 常规说明

  CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].

  This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 纯度

  Protein A purified

• 纯化说明

  Purified from TCS. Purity >95% by SDS-PAGE.

• Primary antibody说明

  CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].

• 克隆

  单克隆

• 克隆编号

  MEM-238

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8093于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3482 Human

• Omim: 147280 Human

• SwissProt: P11717 Human

• Unigene: 487062 Human

• Unigene: 673278 Human

• 别名

• Cation-independent mannose-6-phosphate receptor antibody

• CD222 antibody

• CD222 antigen antibody

• CI Man 6 P receptor antibody

• CI Man-6-P receptor antibody

• CI MPR antibody

• CI-M6PR antibody

• CI-MPR antibody

• CIMPR antibody

• IGF 2 receptor antibody

• IGF 2R antibody

• IGF II receptor antibody

• IGF-II receptor antibody

• IGF2 receptor antibody

• Igf2r antibody

• Insulin like growth factor 2 receptor antibody

• Insulin like growth factor II receptor antibody

• Insulin-like growth factor 2 receptor antibody

• Insulin-like growth factor II receptor antibody

• M6P R antibody

• M6P/IGF2 receptor antibody

• M6P/IGF2R antibody

• M6PR antibody

• mannose 6 phosphate receptor antibody

• mannose 6 phosphate receptor, cation independent antibody

• MPR 300 antibody

• MPR300 antibody

• MPRI antibody

• MPRI_HUMAN antibody

• 300 kDa mannose 6 phosphate receptor antibody

• 300 kDa mannose 6-phosphate receptor antibody

• Cation independent mannose 6 phosphate receptor antibody

• 

  Western blot - Anti-M6PR (cation independent) antibody [MEM-238] (ab8093)

  All lanes : Anti-M6PR (cation independent) antibody [MEM-238] (ab8093) at 1 µg/ml
  
  Lane 1 : Jurkat whole cell extract, with reducing SDS loading buffer
  Lane 2 : K562 whole cell extract, with reducing SDS loading buffer
  Lane 3 : Raji whole cell extract, with reducing SDS loading buffer
  Lane 4 : HeLa whole cell extract, with reducing SDS loading buffer
  Lane 5 : Jurkat whole cell extract, with non-reducing SDS loading buffer
  Lane 6 : K562 whole cell extract, with non-reducing SDS loading buffer
  Lane 7 : Raji whole cell extract, with non-reducing SDS loading buffer
  Lane 8 : HeLa whole cell extract, with non-reducing SDS loading buffer
  
  Secondary
  All lanes : IRDye 800CW Goat-anti-Mouse IgG (green)
  
  Predicted band size: 274 kDa
  
  
  
  

  
  Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, K562, Raji, and HeLa cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis.
  
  Nitrocellulose membrane blot was probed with ab8093 followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.
  
  CD222 molecules were detected at ~250 kDa in all analysed cell lines.

• 

  Flow Cytometry - Anti-M6PR (cation independent) antibody [MEM-238] (ab8093)

  Flow cytometry surface staining analysis of human peripheral whole blood showing separation of human neutrophil granulocytes (red) from lymphocytes black using ab8093 at 2 μg/ml GAM APC.

• 

  Flow Cytometry - Anti-M6PR (cation independent) antibody [MEM-238] (ab8093)

  Flow cytometry analysis of human peripheral whole blood stained using ab8093 in 2 μg/ml and GAM APC.

• 

  Flow Cytometry (Intracellular) - Anti-M6PR (cation independent) antibody [MEM-238] (ab8093)

  Overlay histogram showing HeLa cells stained with ab8093 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8093, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.