Anti-IL-2 Receptor alpha antibody [MEM-181]

• 产品名称

  Anti-IL-2 Receptor alpha抗体[MEM-181]
  参阅全部 IL-2 Receptor alpha 一抗

• 描述

  小鼠单克隆抗体[MEM-181] to IL-2 Receptor alpha

• 宿主

  Mouse

• 特异性

  CD25 antigen (human).

• 经测试应用

  适用于: Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human IL-2 Receptor alpha. PHA-activated peripheral blood leucocytes

• 表位

  extracellular

• 阳性对照

• Flow Cyt: Jurkat cells

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from TCS. Purity >95% by SDS-PAGE.

• 克隆

  单克隆

• 克隆编号

  MEM-181

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab8235于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3559 Human

• Omim: 147730 Human

• SwissProt: P01589 Human

• Unigene: 231367 Human

• 别名

• IDDM10 antibody

• IL 2 receptor alpha subunit antibody

• IL-2 receptor subunit alpha antibody

• IL-2-RA antibody

• IL-2R subunit alpha antibody

• IL2 RA antibody

• IL2 Receptor alpha antibody

• IL2-RA antibody

• IL2R antibody

• IL2R, alpha chain antibody

• IL2RA antibody

• IL2RA_HUMAN antibody

• IMD41 antibody

• Interleukin 2 receptor alpha antibody

• Interleukin 2 receptor antibody

• Interleukin-2 receptor subunit alpha antibody

• p55 antibody

• t-cell growth factor receptor antibody

• TAC antibody

• TAC antigen antibody

• TCGFR antibody

• Interleukin 2 receptor alpha chain antibody

• CD25 antibody

• CD25 antigen antibody

• 

  Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235)

  Flow cytometry analysis showing separation of human PHA stimulated peripheral blood mononuclear cells stained using ab8235 (concentration in sample 2 μg/ml, GAM APC, red-filled) from human PHA stimulated peripheral blood mononuclear cells unstained by primary antibody (GAM APC, black-dashed).

• 

  Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235)

  Overlay histogram showing Jurkat cells stained with ab8235 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8235, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.