Anti-Smac/Diablo抗体
参阅全部 Smac/Diablo 一抗
兔多克隆抗体to Smac/Diablo
Rabbit
适用于: IHC-P, WBmore details
与反应: Mouse, Human
Peptide (
EERAESEQEAYLRED
)corresponding to amino acids 225 to 239 of humanSmac/DIABLO (1).
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
pH: 7.2
Preservative: 0.02% Sodium azide
浓度
100 µg 浓度为 1 mg/ml
DEAE-Chromatography
多克隆
IgG
Abpromise™承诺保证使用ab8114于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa. |
Entrez Gene: 56616 Human
Entrez Gene: 66593 Mouse
Omim: 605219 Human
SwissProt: Q9NR28 Human
SwissProt: Q9JIQ3 Mouse
Unigene: 169611 Human
Unigene: 46716 Mouse
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Western blot - Anti-Smac/Diablo antibody (ab8114)
All lanes : Anti-Smac/Diablo antibody (ab8114) at 1 µg/ml
Lane 1 : human heart tissue lysate with absence of blocking peptide
Lane 2 : human heart tissue lysate with Smac / Diablo peptide (ab8380)
Predicted band size: 25 kDa
Observed band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody (ab8114)
Immunohistochemical analysis of paraffin-embedded human ovary tissue with ab8114 at 5 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-Smac/Diablo antibody (ab8114)
All lanes : Anti-Smac / Diablo antibody (ab8114) at 1 µg/mL, 1h incubation at RT in 5% NFM/TBST.
Loading: 15 μg of lysates per lane.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution.
Western blot - Anti-Smac/Diablo antibody (ab8114)
Anti-Smac/Diablo antibody (ab8114) at 1 µg/ml + NIH3T3 cell lysate at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 25 kDa
