Anti-MTA2/PID抗体
参阅全部 MTA2/PID 一抗
兔多克隆抗体to MTA2/PID
Rabbit
No cross-reactivity to MTA1.
适用于: IHC-Fr, ELISA, WB, IHC-P, ICC/IF, IPmore details
与反应: Mouse, Rat, Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Liquid
Shipped at 4°C. Store at +4°C.
pH: 7.2
Preservative: 0.02% Sodium azide
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab8106于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 18413351 | |
| ELISA | Use at an assay dependent concentration. | |
| WB | (1) | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IP | (1) | Use at an assay dependent concentration. |
Entrez Gene: 9219 Human
Entrez Gene: 23942 Mouse
Omim: 603947 Human
SwissProt: O94776 Human
SwissProt: Q9R190 Mouse
Unigene: 173043 Human
Unigene: 25339 Mouse
Metastasis associated 1 like 1 antibody
Metastasis associated gene 1 like 1 antibody
Metastasis associated gene family member 2 antibody
Metastasis associated protein 2 antibody
Metastasis associated protein MTA 2 antibody
Metastasis associated protein MTA2 antibody
Metastasis-associated 1-like 1 antibody
Metastasis-associated protein MTA2 antibody
Mmta2 antibody
MTA1 L1 protein antibody
MTA1-L1 protein antibody
MTA1L1 antibody
MTA2 antibody
MTA2_HUMAN antibody
p53 target protein in deacetylase complex antibody
PID antibody
DKFZp686F2281 antibody
Mata1l1 antibody
Metastasis associated 1 family member 2 antibody
Western blot - Anti-MTA2/PID antibody (ab8106)
All lanes : Anti-MTA2/PID antibody (ab8106) at 1 µg/ml
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : HeLa whole cell lysate with presence of blocking peptide
Predicted band size: 75 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody (ab8106)
ab8106 at 10µg/ml staining MTA2/PID in Hela cells by ICC/IF
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody (ab8106)
ab8106 (2µg/ml) staining MTA2/PID in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody (ab8106)
Immunofluorescence of PID in HeLa cells using ab8106 at 10 ug/ml.
