Anti-BrdU antibody [IIB5]

• 产品名称

  Anti-BrdU抗体[IIB5]
  参阅全部 BrdU 一抗

• 描述

  小鼠单克隆抗体[IIB5] to BrdU

• 宿主

  Mouse

• 特异性

  BrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating cells. The antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is 100% crossreactive with Iodo-Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used in studies.

• 经测试应用

  适用于: IHC-FoFr, ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Chemical/ Small Molecule corresponding to BrdU conjugated to bovine serum albumin.

• 阳性对照

• Bromodeoxyuridine labeled cells.

• 常规说明

  The following product is available as purified antibody (purified by affinity chromatography) together with several conjugated versions:

  Anti-BrdU antibody [BU1/75 (ICR1)] (ab6326)
  
  

  Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.

   

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

• 存储溶液

  pH: 7.3
  Preservative: 0.09% Sodium azide
  Constituents: 1% BSA, PBS

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  IIB5

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8152于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 细胞定位

  Nuclear

• 别名

• Bromodeoxyuridine antibody

• BUdr antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8152)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

  ab8152 (1/100) staining BrdU in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further esperimental details please see Abreview.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8152)This image is courtesy of an anonymous Abreview

  Immunohistochemical analysis of rat brain tissue, staining BrdU with ab8152.
  
  Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/20 in diluent) for 18 hours at 25°C. A Cy3®-conjugated donkey anti-mouse polyclonal IgG was used as the secondary antibody.

  See Abreview

• 

  Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8152)

  Cells were pulse labeled with 10 µM BrdU for 30 min, rinsed twice in prewarmed PBS, and chased in prewarmed culture medium, supplemented with 5 mM deoxythymidine. Incorporated BrdU was detected after ethanol fixation of the cells, which were than rinsed once in PBS and resuspended in 2 ml of 0.4 mg/ml pepsin in 0.1 N HCl. After 30 min at room temperature cells were pelleted, resuspended in 2 N HCl, and incubated for another 30 min at 37°C. Cells were rinsed in 0.1 M borate buffer, pH 8.5, and PBS/BSA (1 mg/ml BSA in PBS). Appropriately diluted mouse anti-BrdU antibody (clone IIB5) was added to the cell pellet, resuspended in 100 micro liters PBS/BSA. After incubation for 1 h at room temperature, the cells were rinsed twice in PBS/BSA. For visualization, FITC-conjugated Fab2 fragments of rabbit anti-mouse Ig antibody were added in a 1/10 dilution. After incubation for 45 min at room temperature samples were rinsed twice in PBS/BSA and the cells were finally resuspended in 0.5 ml cold PBS supplemented with 100 microgram/ml RNAse and 20 µg/mL propidium iodide. The samples were allowed to stand for 15 min on ice in the dark before flow cytometric analysis. In the negative control the primary antibody was omitted.