Anti-CD3 antibody [MEM-57]

• 产品名称

  Anti-CD3抗体[MEM-57]
  参阅全部 CD3 一抗

• 描述

  小鼠单克隆抗体[MEM-57] to CD3

• 宿主

  Mouse

• 特异性

  This antibody reacts with epsilon-gamma and epsilon-delta dimers of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor for antigen (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. It reacts more strongly with blood gamma-delta T lymphocytes than with alpha-beta T lymphocytes.

• 经测试应用

  适用于: Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human CD3. Human thymocytes and T lymphocytes

• 常规说明

  Activates T cells in vitro. This antibody is a highly effective immunosuppressive agent. In this it is similar to OKT3.

  
  

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  This product has been purified from tissue culture supernatant.

• Primary antibody说明

  Activates T cells in vitro. This antibody is a highly effective immunosuppressive agent. In this it is similar to OKT3.

• 克隆

  单克隆

• 克隆编号

  MEM-57

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab8090于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

  Human peripheral blood lymphocytes stained with ab8090 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

• 

  Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

  Flow cytometry analysis of human peripheral whole blood labeling CD3 with ab8090 at 0.33 μg/mL (GAM APC).

• 

  Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

  Flow cytometry analysis of human peripheral whole blood labeling CD3 with ab8090 at 0.33 μg/mL (GAM APC). Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).

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  Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

  Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD3 (MEM-57) Dy161. Gated on singlets.