Anti-Endothelium抗体[EN4]
参阅全部 Endothelium 一抗
小鼠单克隆抗体[EN4] to Endothelium
Mouse
This antibody stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen (ab8086) which is lost. It stains strongly murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.
适用于: IHC-Fr, IHC-P, WBmore details
与反应: Guinea pig, Cat, Human
不与反应: Mouse, Sheep, Rabbit, Cow, Dog
The antibody is useful for immunohistological staining of endothelia. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
pH: 7.3
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA
Tissue culture supernatant
The antibody is useful for immunohistological staining of endothelia. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained.
单克隆
EN4
IgG1
Abpromise™承诺保证使用ab8087于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. | |
WB | Use at an assay dependent concentration. PubMed: 21645391 |