Anti-TNF Receptor II antibody [MR2-1]

• 产品名称

  Anti-TNF Receptor II抗体[MR2-1]
  参阅全部 TNF Receptor II 一抗

• 描述

  小鼠单克隆抗体[MR2-1] to TNF Receptor II

• 宿主

  Mouse

• 特异性

  The antibody reacts with the extra-cellular part of the TNF-R II and with the soluble receptor.

• 经测试应用

  适用于: ICC/IF, Flow Cytmore details

• 种属反应性

  与反应: Human
  预测可用于: Cynomolgus monkey, Rhesus monkey

• 免疫原

  Recombinant fragment corresponding to Human TNF Receptor II (extracellular). Soluble TNF-R75 from culture supernatant of cells transfected with the extracellular part of TNF-R75, purified by affinity chromatography on a TNF-coupled agarose column.
  Database link: P20333

• 阳性对照

• Recombinant human Soluble TNF Receptor II protein (ab640) can be used as a positive control in WB.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.02% Sodium azide

• 浓度

• 500 µl 浓度为 0.1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  MR2-1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8161于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7133 Human

• Omim: 191191 Human

• SwissProt: P20333 Human

• Unigene: 256278 Human

• 别名

• p75TNFR antibody

• p80 TNF alpha receptor antibody

• p80 TNF-alpha receptor antibody

• Soluble TNFR1B variant 1 antibody

• TBP-2 antibody

• TBPII antibody

• TNF R II antibody

• TNF R2 antibody

• TNF R75 antibody

• TNF-R2 antibody

• TNF-RII antibody

• TNFBR antibody

• TNFR-II antibody

• TNFR1B antibody

• TNFR2 antibody

• TNFR80 antibody

• TNFRII antibody

• Tnfrsf1b antibody

• TNR1B_HUMAN antibody

• Tumor necrosis factor beta receptor antibody

• Tumor necrosis factor binding protein 2 antibody

• Tumor necrosis factor receptor 2 antibody

• Tumor necrosis factor receptor superfamily member 1B antibody

• Tumor necrosis factor receptor type II antibody

• Tumor necrosis factor-binding protein 2 antibody

• CD120b antibody

• p75 antibody

• p75 TNF receptor antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor II antibody [MR2-1] (ab8161)

  ICC/IF image of ab8161 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Flow Cytometry - Anti-TNF Receptor II antibody [MR2-1] (ab8161)

  Overlay histogram showing HL60 cells stained with ab8161 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8161, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.