Anti-pan Keratin antibody [80]

• 产品名称

  Anti-pan Keratin抗体[80]
  参阅全部 pan Keratin 一抗

• 描述

  小鼠单克隆抗体[80] to pan Keratin

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, IHC-Fr, IHC-P, WB, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Full length native protein (purified) corresponding to Human pan Keratin. Callus cytokeratins isolated from fresh human skin tissue

• 阳性对照

• Human callus or keratinocytes

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.07% Sodium azide
  Constituent: 1% Fetal calf serum

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  80

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8068于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3848 Human

• Omim: 139350 Human

• SwissProt: P04264 Human

• SwissProt: P04104 Mouse

• 别名

• K1 antibody

• Keratin 1 antibody

• KRT1 antibody

• KRT1A antibody

• KRTA antibody

• CK1 antibody

• Cytokeratin 1 antibody

• EHK1 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)

  ICC/IF image of ab8068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-pan Keratin antibody [80] (ab8068)This image is courtesy of an anonymous Abreview

  Ab8068 staining pan Keratin in Human glioblastoma cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with paraformaldehyde; permeabilized with 0.1% Triton X 100 in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 dilution in 0.5% BSA in PBS) for 16hours at 4°C. An Alexa Fluor® 488 anti-mouse was used as a secondary antibody at 1/400 dilution.

  See Abreview

• 

  Western blot - Anti-pan Keratin antibody [80] (ab8068)

  Anti-pan Keratin antibody [80] (ab8068) at 1/250 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 65 kDa
  Observed band size: 65 kDa
  Additional bands at: 49 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 30 seconds
  

• 

  Flow Cytometry - Anti-pan Keratin antibody [80] (ab8068)

  Overlay histogram showing A431 cells stained with ab8068 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8068, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.