Anti-Collagen IV抗体
参阅全部 Collagen IV 一抗
兔多克隆抗体to Collagen IV
Rabbit
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
适用于: ELISA, IHC-Fr, WB, IHC-P, IP, ICC/IF, IHC-FrFl, IHC-FoFrmore details
与反应: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Zebrafish, African green monkey, Chinese hamster, Syrian hamster
预测可用于: Mammals
This product was produced with the following immunogens:
Full length native protein (purified) corresponding to Human Collagen IV. Collagen Type IV from human and bovine placenta. The immunogen maintains the native conformation of the protein.
Database link: P02462
Full length native protein (purified) corresponding to Bovine Collagen IV.
Database link: Q7SIB2
IHC-P: Human kidney, liver, lung, renal oncocytoma and skeletal muscle tissues.
There are other recombinant monoclonal options, such as Recombinant Anti-Collagen IV antibody ab214417.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
批次浓度范围 100 µg 浓度为 1 - 1.17 mg/ml
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
多克隆
IgG
Abpromise™承诺保证使用ab6586于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | (21) | Use at an assay dependent concentration. |
| WB | (26) | Use at an assay dependent concentration. Predicted molecular weight: 161 kDa. This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
| IHC-P | (36) | 1/15 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| IP | (3) | Use at an assay dependent concentration. |
| ICC/IF | (23) | Use at an assay dependent concentration. PubMed: 19933193 |
| IF | Use at an assay dependent concentration. | |
| IHC-FrFl | (1) | Use at an assay dependent concentration. |
| IHC-FoFr | (8) | Use at an assay dependent concentration. |
Entrez Gene: 1282 Human
Entrez Gene: 1284 Human
Entrez Gene: 1285 Human
Entrez Gene: 1286 Human
Entrez Gene: 1287 Human
Entrez Gene: 12826 Mouse
Entrez Gene: 12827 Mouse
Entrez Gene: 12828 Mouse
Entrez Gene: 12829 Mouse
Entrez Gene: 12830 Mouse
Entrez Gene: 323561 Zebrafish
Entrez Gene: 554269 Zebrafish
Entrez Gene: 554270 Zebrafish
Omim: 120070 Human
Omim: 120090 Human
Omim: 120130 Human
Omim: 120131 Human
Omim: 303630 Human
SwissProt: P02462 Human
SwissProt: P08572 Human
SwissProt: P29400 Human
SwissProt: P53420 Human
SwissProt: Q01955 Human
SwissProt: P02463 Mouse
SwissProt: P08122 Mouse
SwissProt: Q9QZR9 Mouse
SwissProt: Q9QZS0 Mouse
Unigene: 17441 Human
Unigene: 738 Mouse
Arresten antibody
BSVD antibody
CO4A1_HUMAN antibody
COL4A1 antibody
COL4A1 NC1 domain antibody
COL4A2 antibody
COL4A3 antibody
COL4A4 antibody
COL4A5 antibody
collagen alpha-1(IV) chain antibody
Collagen IV Alpha 1 Polypeptide antibody
Collagen IV Alpha 2 Polypeptide antibody
Collagen Of Basement Membrane Alpha 1 Chain antibody
Collagen Of Basement Membrane Alpha 2 Chain antibody
Collagen Type IV Alpha 1 antibody
collagen type IV alpha 1 chain antibody
Collagen Type IV Alpha 2 antibody
Collagen Type IV Alpha 3 antibody
Collagen Type IV Alpha 4 antibody
Collagen Type IV Alpha 5 antibody
RATOR antibody
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Collagen IV antibody (ab6586)This image is courtesy of an anonymous Abreview
Immunohistochemistry analysis of paraformaldehyde perfusion fixed frozen pig kidney permeabilized with 0.05% tween 20. Stained with ab6586 at 1/200 dilution. Secondary antibody used was Alexa Fluor® 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed at 1/300 dilution. The nuclei are stained Blue with Hoechst. The Red counter stain was created with a methyl green stain and by imaging with a 555nm excitation filter. Blocking was done with Sea Block for 30 minutes at 22°C.
The sample was incubated with the primary antibody and Sea Block for 16 hours at 4°C. Antigen retrieval method was heat mediated with Citrate Buffer pH 6.0.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde fixed human brain (cortex) staining with ab6586 at 1/250 dilution. Secondary antibody used was HRP Goat Agilent anti-mouse/rabbit EnVision Dual Link.
Blocking was done using 5% serum for 15 minutes at 20°C. The sample was incubated with the primary antibody for 1 hour at 20°C with Agilent antibody diluent. Antigen retrieval method was heat mediated Citrate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Immunohistochemical analysis formalin-fixed paraffin-embedded human lung tissue labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: basement membranes and vessels. Counterstained with hematoxylin for 15 seconds.
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human skeletal muscle cells labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: cells surrounded by collagen IV fibers. Counterstained with hematoxylin for 15 seconds.
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human renal oncocytoma tissue labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: dense collagen IV positive membranes surrounding tumor cell nests. Counterstained with hematoxylin for 15 seconds
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Paraffin-embedded human kidney tissue stained for Collagen IV using ab6586 at 1/400 dilution in immunohistochemical analysis with strong staining observed in glomeruli.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Paraffin-embedded human liver tissue stained for Collagen IV using ab6586 at 1/400 dilution in immunohistochemical analysis, strong staining was observed in the sinusoids.
