Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)

• 产品名称

  兔抗大鼠IgG H&L (Alkaline Phosphatase)
  参阅全部 IgG 二抗

• 宿主

  Rabbit

• 靶标种属

  Rat

• 经测试应用

  适用于: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details

• 免疫原

  Rat IgG whole molecule

• 偶联物

  Alkaline Phosphatase

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 8
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride

• 浓度

• 1 mg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 纯化说明

  Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

• 共轭说明

  Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

• 克隆

  多克隆

• 常规说明

  Part of the AbExcel range.

The Abpromise guarantee

Abpromise™承诺保证使用ab6735于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735)

  All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
  
  All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Lanes 1-2 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/2000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
  Lanes 3-4 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/10000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
  Lanes 5-6 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.