生物素Anti-Collagen IV抗体
参阅全部 Collagen IV 一抗
生物素兔多克隆抗体to Collagen IV
Rabbit
Biotin
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
适用于: ELISA, WB, IP, IHC-Pmore details
与反应: Cow, Human
预测可用于: Mammals
Full length native protein (purified) corresponding to Collagen IV. Collagen Type IV from human and bovine placenta.
At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
多克隆
IgG
Abpromise™承诺保证使用ab6581于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | (1) | Use at an assay dependent concentration. |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 161 kDa. Not recommended for use under denaturing conditions. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | 1/1000 - 1/5000. |
Entrez Gene: 1282 Human
Entrez Gene: 1284 Human
Entrez Gene: 1285 Human
Entrez Gene: 1286 Human
Entrez Gene: 1287 Human
Omim: 120070 Human
Omim: 120090 Human
Omim: 120130 Human
Omim: 120131 Human
Omim: 303630 Human
SwissProt: P02462 Human
SwissProt: P08572 Human
SwissProt: P29400 Human
SwissProt: P53420 Human
SwissProt: Q01955 Human
Unigene: 17441 Human
Arresten antibody
BSVD antibody
CO4A1_HUMAN antibody
COL4A1 antibody
COL4A1 NC1 domain antibody
COL4A2 antibody
COL4A3 antibody
COL4A4 antibody
COL4A5 antibody
collagen alpha-1(IV) chain antibody
Collagen IV Alpha 1 Polypeptide antibody
Collagen IV Alpha 2 Polypeptide antibody
Collagen Of Basement Membrane Alpha 1 Chain antibody
Collagen Of Basement Membrane Alpha 2 Chain antibody
Collagen Type IV Alpha 1 antibody
collagen type IV alpha 1 chain antibody
Collagen Type IV Alpha 2 antibody
Collagen Type IV Alpha 3 antibody
Collagen Type IV Alpha 4 antibody
Collagen Type IV Alpha 5 antibody
RATOR antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Collagen IV antibody (ab6581)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human tissue sections, labelling Collagen IV with ab6581 at a concentration of 10 µg/mL for 1 hour at room temperature. The left panel is human kidney sections with the right panel being human liver sections. Antigen retrival was performed with 0.01 M sodium citrate buffer at pH 6.0 at 99°C for 20 mins. The secondary used was a rabbit peroxidase secondary antibody at a 1/10,000 dilution incubated for 45 mins at room temperature. Counterstaining against nuclear DNA was hematoxylin.
