驴抗山羊IgG H&L (Cy5 ®)预吸附二抗
参阅全部 IgG 二抗
Donkey
Goat
适用于: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details
Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Ratmore details
Goat IgG, whole molecule
Cy5 ®. Ex: 650nm, Em: 667nm
Liquid
Shipped at 4°C. Store at +4°C.
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
浓度
500 µg 浓度为 1 mg/ml
Immunogen affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
多克隆
IgG
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
Abpromise™承诺保证使用ab6566于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent dilution. | |
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | (1) | Use at an assay dependent dilution. |
| ELISA | 1/10000 - 1/50000. |
Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)Li Q et al. ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex. PLoS One 8:e65703 (2013).
Embryonic brains were fixed in 4% paraformaldehyde in PBS overnight and placed in 15% sucrose/PBS for 12 h and then 30% sucrose/PBS for 24 h at 4°C. Brains were then embedded in OCT (optimum cutting temperature) mounting medium and froze before sectioning at 10 or 20 µm. Embryonic cerebral cortex from E14.5, E15.5, E16.5 and E18.5 mice were dissected out and sectioned at 10–15 µm for immunohistochemistry experiment with anti-ADAM17 (ab13535) antibody.
Cy5-labeled donkey anti-goat (ab6566) was used as the secondary antibody (Red).
Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)This image is courtesy of an anonymous Abreview
Lanes 2-3 : Goat anti-UCP1 at 1/200 dilution
Lane 1 : Marker
Lanes 2-3 : Mouse brown adipose tissue lysate at 70 µg
Secondary
Lanes 2-3 : Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution
Observed band size: 33 kDawhy is the actual band size different from the predicted?
