Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]

• 产品名称

  抗单纯疱疹病毒1 ICP4 Immediate Early蛋白抗体[10F1]

• 描述

  小鼠单克隆抗体[10F1] to单纯疱疹病毒1 ICP4 Immediate Early蛋白

• 宿主

  Mouse

• 特异性

  ELISA titer 1/25,600 absorption of 0.10

• 经测试应用

  适用于: WB, ELISA, ICC/IFmore details

• 种属反应性

  与反应: Herpes simplex virus
  

• 免疫原

  Tissue, cells or virus corresponding to HSV 1 ICP4 Immediate Early Protein.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.4

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  10F1

• 骨髓瘤

  NS1/1-Ag4-1

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab6514于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2703392 Herpes simplex virus

• SwissProt: P08392 Herpes simplex virus

• 别名

• Immediate early protein antibody

• RS1 antibody

• Transcriptional regulator ICP4 antibody

• Herpes simplex virus 1 ICP4 antibody

• HSV RS1 antibody

• ICP4 antibody

• 

  Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)

  Lane 1 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/200 dilution
  Lane 2 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/400 dilution
  Lane 3 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/800 dilution
  Lane 4 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1600 dilution
  
  All lanes : BCIP/NBT. HSV-1 MacIntyre/Vero infected cell extract
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Anti-Mouse secondary at 1/5000 dilution
  

• 

  Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)This image is courtesy of an anonymous Abreview

  All lanes : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1000 dilution
  
  Lane 1 : HeLa cells (whole cell lysate) - mock infection, cytosolic fraction
  Lane 2 : HeLa cells (whole cell lysate) - mock infection, membrane fraction
  Lane 3 : HeLa cells (whole cell lysate) - mock infection, nuclear fraction
  Lane 4 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - cytosolic fraction
  Lane 5 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours -membrane fraction
  Lane 6 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - nuclear fraction
  Lane 7 : HeLa cells (whole cell lysate) - mock infected
  Lane 8 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : HRP conjugated sheep anti-mouse IgG
  
  Observed band size: 97 kDawhy is the actual band size different from the predicted?
  
  
  
  As shown, ICP4 is detected mainly in the nuclear fraction of infected cells (lane 6). The presence of ICP4 in the cytosolic fraction (lane 4) probably represents the protein undergoing synthesis.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)This image is courtesy of an anonymous Abreview

  ab6514 (1/5000) staining HSV1 ICP4 immediate Early Protein in 293T cells transiently transfected with a plasmid expressing HSV ICP4 (green). Cells were formaldehyde-fixed and incubated with the primary antibody for 1 hour. A FITC conjugated rabbit polyclonal antibody was used as the secondary antibody.

  See Abreview