Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

• 产品名称

  Anti-PSD95抗体[6G6-1C9] - Synaptic Marker
  参阅全部 PSD95 一抗

• 描述

  小鼠单克隆抗体[6G6-1C9] to PSD95 - Synaptic Marker

• 宿主

  Mouse

• 特异性

  We do not guarantee IHC-P for mouse.

• 经测试应用

  适用于: IHC-P, WBmore details

• 种属反应性

  与反应: Mouse, Rat
  预测可用于: Cow, Zebrafish

• 免疫原

  Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• IHC-P: Rat retina tissue. WB: Rat and mouse hippocampus and forebrain tissue lysate.

• 常规说明

  The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  6G6-1C9

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab2723于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 100137840 Cow

• Entrez Gene: 13385 Mouse

• Entrez Gene: 29495 Rat

• Entrez Gene: 405796 Zebrafish

• SwissProt: Q62108 Mouse

• SwissProt: P31016 Rat

• SwissProt: Q6R005 Zebrafish

• Unigene: 27256 Mouse

• Unigene: 9765 Rat

• Unigene: 30412 Zebrafish

别名

• Discs large homolog 4 antibody

• Disks large homolog 4 antibody

• DLG 4 antibody

• Dlg4 antibody

• DLG4_HUMAN antibody

• FLJ97752 antibody

• FLJ98574 antibody

• Human post synaptic density protein 95 antibody

• Post synaptic density protein 95 antibody

• Postsynaptic density protein 95 antibody

• PSD 95 antibody

• PSD-95 antibody

• PSD95 antibody

• SAP 90 antibody

• SAP-90 antibody

• SAP90 antibody

• Synapse associated protein 90 antibody

• Synapse-associated protein 90 antibody

• Tax interaction protein 15 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)

  IHC image of ab2723 staining in formalin-fixed, paraffin-embedded rat retinal tissue, performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2723, 2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

  Secondary-only control image is shown as insert.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)

  All lanes : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 5 µg/ml
  
  Lane 1 : Hippocampus (Rat) Tissue Lysate
  Lane 2 : Forebrain (Rat) Tissue Lysate
  Lane 3 : Hippocampus (Mouse) Tissue Lysate
  Lane 4 : Forebrain (Mouse) Tissue Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 80 kDa
  Observed band size: 95 kDawhy is the actual band size different from the predicted?
  Additional bands at: 100 kDa, 110 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 3 minutes
  

• 

  Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)Image courtesy of an AbReview submitted by Grant Corbett

  Lanes 1-6 : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 1/1200 dilution
  Lanes 7-12 : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 1/1200 dilution (18 hours at 4°C)
  
  Lanes 1-6 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
  Lanes 7-12 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Li-Cor IRDye® Donkey anti-mouse 680LT at 1/18000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 80 kDa
  Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 5 minutes
  

  See Abreview