Anti-Anillin抗体
参阅全部 Anillin 一抗
山羊多克隆抗体to Anillin
Goat
适用于: Flow Cyt (Intra), ICC/IF, IHC-Pmore details
与反应: Human
预测可用于: Mouse, Cow
Synthetic peptide corresponding to Human Anillin aa 1100 to the C-terminus (C terminal). (NP_001271230.1; NP_001271231.1)
Database link: Q9NQW6
ICC/IF: U2OS cells. Flow Cyt (intra): MCF7 cells. IHC-P: Human kidney tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab5910于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) | Use a concentration of 10 µg/ml. | |
| ICC/IF | (3) | Use a concentration of 10 µg/ml. |
| IHC-P | (1) | Use a concentration of 3 - 10 µg/ml. |
Entrez Gene: 54443 Human
Entrez Gene: 68743 Mouse
Omim: 616027 Human
SwissProt: Q9NQW6 Human
SwissProt: Q8K298 Mouse
Unigene: 62180 Human
Unigene: 282751 Mouse
Unigene: 453238 Mouse
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody (ab5910)
ab5910 at 10ug/ml staining Anillin in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9.0). HRP-staining procedure was used for detection.
Flow Cytometry (Intracellular) - Anti-Anillin antibody (ab5910)
Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) and permeabilized with 0.5% Triton using ab5910 . Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) fol
Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody (ab5910)
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Anillin with ab5910 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody (ab5910)This image is courtesy of an Abreview submitted by Manoj B Menon
ab14404 staining Anillinin Mouse fibroblast cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 4% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/25 in PBS + 1% BSA) for 2 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-goat polyclonal(1/500) was used as the secondary antibody.
