Anti-NCOR2/SMRT抗体
参阅全部 NCOR2/SMRT 一抗
兔多克隆抗体to NCOR2/SMRT
Rabbit
ab5802 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human cells.
适用于: ICC/IF, IHC-Pmore details
与反应: Mouse, Human
Recombinant fragment within Human NCOR2/SMRT aa 50-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
多克隆
IgG
Abpromise™承诺保证使用ab5802于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/100 - 1/200. | |
| IHC-P | 1/100 - 1/1000. |
Entrez Gene: 9612 Human
Entrez Gene: 20602 Mouse
Omim: 600848 Human
SwissProt: Q9Y618 Human
SwissProt: Q9WU42 Mouse
Unigene: 137510 Human
Unigene: 278646 Mouse
N CoR2 antibody
N-CoR2 antibody
NCoR 2 antibody
Ncor2 antibody
NCOR2_HUMAN antibody
Nuclear Receptor Co-Repressor 2 antibody
Nuclear receptor corepressor 2 antibody
retinoic-acid-receptor-associated corepressor antibody
Silencing Mediator for Retanoid and Thyroid Hormone Receptors antibody
Silencing mediator of retinoic acid and thyroid hormone receptor antibody
SMAP 270 antibody
SMAP270 antibody
SMRT antibody
SMRTE antibody
SMRTE tau antibody
T3 receptor associating factor antibody
T3 receptor-associating factor antibody
Thyroid retinoic acid receptor associated corepressor antibody
Thyroid- antibody
TNRC 14 antibody
TNRC14 antibody
TRAC 1 antibody
TRAC antibody
TRAC1 antibody
CTG 26 antibody
CTG repeat protein 26 antibody
CTG26 antibody
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (ab5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (ab5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (ab5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
Immunocytochemistry/ Immunofluorescence - Anti-NCOR2/SMRT antibody (ab5802)
Immunofluorescent analysis of NCOR2/SMRT (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOR2/SMRT antibody (ab5802)
ab5802 labelling NCOR2/SMRT in mouse brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). Antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min and tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with primary antibody (1:500 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOR2/SMRT antibody (ab5802)
ab5802 labelling NCOR2/SMRT in human lung tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). Antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min and tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with primary antibody (1:500 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
