Anti-PHAP1抗体
参阅全部 PHAP1 一抗
兔多克隆抗体to PHAP1
Rabbit
This antibody does not cross-react with PHAP12a or PHAPIII.
适用于: ICC/IF, WBmore details
与反应: Human
预测可用于: Rat
Synthetic peptide corresponding to Human PHAP1.
(Peptide available as ab6241)
WB: Daudi, HeLa, HepG2, K562 and Raji cell lysates. ICC/IF: Raji cells
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS
DEAE-Chromatography
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.
多克隆
IgG
Abpromise™承诺保证使用ab5992于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 2 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. |
Entrez Gene: 8125 Human
Omim: 600832 Human
SwissProt: P39687 Human
Unigene: 458747 Human
Unigene: 10123 Rat
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PHAPI antibody
Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
PP32 antibody
Putative HLA DR associated protein I antibody
Putative HLA-DR-associated protein I antibody
Putative human HLA class II associated protein I antibody
Putative human HLA class II-associated protein antibody
acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
Acidic nuclear phosphoprotein 32 family member A antibody
Western blot - Anti-PHAP1 antibody (ab5992)
All lanes : Anti-PHAP1 antibody (ab5992) at 2 µg/ml
Lane 1 : Daudi cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : HepG2 cell lysates
Lane 4 : K562 cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
Immunofluorescent analysis of 4% paraformaldehydefixed Raji Cells labeling PHAP I with ab5992 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Western blot - Anti-PHAP1 antibody (ab5992)
Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively. Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively.
Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
Immunocytochemical analysis of Raji cells labeling PHAP1 with ab5992 at 2 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
